LOCUS       BBa_K1333318            1443 bp    DNA     linear       14-May-2024
DEFINITION  PIII with an RNA thermometer(Rose42)
ACCESSION   BBa_K1333318
VERSION     BBa_K1333318.1  
FEATURES             Location/Qualifiers
     RBS             90..95
                     /label=SD
     misc_feature    74..79
                     /label=Biobrick-Scar-adaptation
     stem_loop       1..15
                     /label=Predicted Stem Loop
     stem_loop       16..36
                     /label=Predicted Stem Loop
     stem_loop       39..71
                     /label=Predicted Stem Loop
     stem_loop       72..96
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    1..96
                     /label=BBa_K115001
     CDS             103..1410
                     /label=g3p with an 12 AAs peptide insertion designed for 
                     phage display
     CDS             1417..1443
                     /label=FLAG
     CDS             153..189
                     /label=12 AAs peptide insertion designed for phage display
     CDS             103..1443
                     /label=BBa_K1333002
ORIGIN
        1 gccgcgacaa gcggtccggg cgccctaggg gcccggcgga gacgggcgcc ggaggtgtcc
       61 gacgcctgct cgtcaagttc ttgctccttg gaggattact agatgaaaaa attattattc
      121 gcaattcctt tagtggtacc tttctattct cactctacta attataatta tgggcctggt
      181 ggaggttcgg ccgaaactgt tgaaagttgt ttagcaaaat cccatacaga aaattcattt
      241 actaacgtct ggaaagacga caaaacttta gatcgttacg ctaactatga gggctgtctg
      301 tggaatgcta caggcgttgt agtttgtact ggtgacgaaa ctcagtgtta cggtacatgg
      361 gttcctattg ggcttgctat ccctgaaaat gagggtggtg gctctgaggg tggcggttct
      421 gagggtggcg gttctgaggg tggcggtact aaacctcctg agtacggtga tacacctatt
      481 ccgggctata cttatatcaa ccctctcgac ggcacttatc cgcctggtac tgagcaaaac
      541 cccgctaatc ctaatccttc tcttgaggag tctcagcctc ttaatacttt catgtttcag
      601 aataataggt tccgaaatag gcagggggca ttaactgttt atacgggcac tgttactcaa
      661 ggcactgacc ccgttaaaac ttattaccag tacactcctg tatcatcaaa agccatgtat
      721 gacgcttact ggaacggtaa attcagagac tgcgctttcc attctggctt taatgaggat
      781 ttatttgttt gtgaatatca aggccaatcg tctgacctgc ctcaacctcc tgtcaatgct
      841 ggcggcggct ctggtggtgg ttctggtggc ggctctgagg gtggtggctc tgagggtggc
      901 ggttctgagg gtggcggctc tgagggaggc ggttccggtg gtggctctgg ttccggtgat
      961 tttgattatg aaaagatggc aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa
     1021 aacgcgctac agtctgacgc taaaggcaaa cttgattctg tcgctactga ttacggtgct
     1081 gctatcgatg gtttcattgg tgacgtttcc ggccttgcta atggtaatgg tgctactggt
     1141 gattttgctg gctctaattc ccaaatggct caagtcggtg acggtgataa ttcaccttta
     1201 atgaataatt tccgtcaata tttaccttcc ctccctcaat cggttgaatg tcgccctttt
     1261 gtctttggcg ctggtaaacc atatgaattt tctattgatt gtgacaaaat aaacttattc
     1321 cgtggtgtct ttgcgtttct tttatatgtt gccaccttta tgtatgtatt ttctacgttt
     1381 gctaacatac tgcgtaataa ggagtctagg tcatcagact acaaagatga cgacgataaa
     1441 taa
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