LOCUS       BBa_K1339004            2407 bp    DNA     linear       14-May-2024
DEFINITION  RBS-otsB-RBS-otsA-dT
ACCESSION   BBa_K1339004
VERSION     BBa_K1339004.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..819
                     /label=BBa_K1339001
     misc_feature    832..835
                     /label=conserved
     RBS             828..839
                     /label=BBa_B0034
     CDS             846..2270
                     /label=BBa_K1339000
     stem_loop       2290..2333
                     /label=stem_loop
     misc_feature    2279..2358
                     /label=BBa_B0010
     terminator      2279..2358
                     /label=BBa_B0010
     stem_loop       2374..2393
                     /label=T7 TE
     misc_feature    2400..2400
                     /label=stop
     polyA_site      2394..2407
                     /label=polya
     misc_feature    2367..2407
                     /label=BBa_B0012
     terminator      2367..2407
                     /label=BBa_B0012
     terminator      2279..2407
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gacagaaccg ttaaccgaaa cccctgaact atccgcgaaa
       61 tatgcctggt tttttgatct tgatggaacg ctggcggaaa tcaaaccgca tcccgatcag
      121 gtcgtcgtgc ctgacaatat tctgcaagga ctacagctac tggcaaccgc aagtgatggt
      181 gcattggcat tgatatcagg gcgctcaatg gtggagcttg acgcactggc aaaaccttat
      241 cgcttcccgt tagcgggcgt gcatggggcg gagcgccgtg acatcaatgg taaaacacat
      301 atcgttcatc tgccggatgc gattgcgcgt gatattagcg tgcaactgca tacagtcatc
      361 gctcagtatc ccggcgcgga gctggaggcg aaagggatgg cttttgcgct gcattatcgt
      421 caggctccgc agcatgaaga cgcattaatg acattagcgc aacgtattac tcagatctgg
      481 ccacaaatgg cgttacagca gggaaagtgt gttgtcgaga tcaaaccgag aggtaccagt
      541 aaaggtgagg caattgcagc ttttatgcag gaagctccct ttatcgggcg aacgcccgta
      601 tttctgggcg atgatttaac cgatgaatct ggcttcgcag tcgttaaccg actgggcgga
      661 atgtcagtaa aaattggcac aggtgcaact caggcatcat ggcgactggc gggtgtgccg
      721 gatgtctgga gctggcttga aatgataacc accgcattac aacaaaaaag agaaaataac
      781 aggagtgatg actatgagtc gtttagtcgt agtatctaat actagagaaa gaggagaaat
      841 actagatgag tcgtttagtc gtagtatcta accggattgc accaccagac gagcacgccg
      901 ccagtgccgg tggccttgcc gttggcatac tgggggcact gaaagccgca ggcggactgt
      961 ggtttggctg gagtggtgaa acagggaatg aggatcagcc gctaaaaaag gtgaaaaaag
     1021 gtaacattac gtgggcctct tttaacctca gcgaacagga ccttgacgaa tactacaacc
     1081 aattctccaa tgccgttctc tggcccgctt ttcattatcg gctcgatctg gtgcaatttc
     1141 agcgtcctgc ctgggacggc tatctacgcg taaatgcgtt gctggcagat aaattactgc
     1201 cgctgttgca agacgatgac attatctgga tccacgatta tcacctgttg ccatttgcgc
     1261 atgaattacg caaacgggga gtgaataatc gcattggttt ctttctgcat attcctttcc
     1321 cgacaccgga aatcttcaac gcgctgccga catatgacac cttgcttgaa cagctttgtg
     1381 attatgattt gctgggtttc cagacagaaa acgatcgtct ggcgttcctg gattgtcttt
     1441 ctaacctgac ccgcgtcacg acacgtagcg caaaaagcca tacagcctgg ggcaaagcat
     1501 ttcgaacaga agtctacccg atcggcattg aaccgaaaga aatagccaaa caggctgccg
     1561 ggccactgcc gccaaaactg gcgcaactta aagcggaact gaaaaacgta caaaatatct
     1621 tttctgtcga acggctggat tattccaaag gtttgccaga gcgttttctc gcctatgaag
     1681 cgttgctgga aaaatatccg cagcatcatg gtaaaattcg ttatacccag attgcaccaa
     1741 cgtcgcgtgg tgatgtgcaa gcctatcagg atattcgtca tcagctcgaa aatgaagctg
     1801 gacgaattaa tggtaaatac gggcaattag gctggacgcc gctttattat ttgaatcagc
     1861 attttgaccg taaattactg atgaaaatat tccgctactc tgacgtgggc ttagtgacgc
     1921 cactgcgtga cgggatgaac ctggtagcaa aagagtatgt tgctgctcag gacccagcca
     1981 atccgggcgt tcttgttctt tcgcaatttg cgggagcggc aaacgagtta acgtcggcgt
     2041 taattgttaa cccctacgat cgtgacgaag ttgcagctgc gctggatcgt gcattgacta
     2101 tgtcgctggc ggaacgtatt tcccgtcatg cagaaatgct ggacgttatc gtgaaaaacg
     2161 atattaacca ctggcaggag tgcttcatta gcgacctaaa gcagatagtt ccgcgaagcg
     2221 cggaaagcca gcagcgcgat aaagttgcta cctttccaaa gcttgcgtag tactagagcc
     2281 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2341 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2401 gtttata
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