LOCUS       BBa_K1339014            2450 bp    DNA     linear       14-May-2024
DEFINITION  Pconst(high)-RBS-otsB-RBS-otsA-dT
ACCESSION   BBa_K1339014
VERSION     BBa_K1339014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..862
                     /label=BBa_K1339001
     misc_feature    875..878
                     /label=conserved
     RBS             871..882
                     /label=BBa_B0034
     CDS             889..2313
                     /label=BBa_K1339000
     stem_loop       2333..2376
                     /label=stem_loop
     misc_feature    2322..2401
                     /label=BBa_B0010
     terminator      2322..2401
                     /label=BBa_B0010
     stem_loop       2417..2436
                     /label=T7 TE
     misc_feature    2443..2443
                     /label=stop
     polyA_site      2437..2450
                     /label=polya
     misc_feature    2410..2450
                     /label=BBa_B0012
     terminator      2410..2450
                     /label=BBa_B0012
     terminator      2322..2450
                     /label=BBa_B0015
     misc_feature    44..2450
                     /label=BBa_K1339004
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgacagaa ccgttaaccg aaacccctga actatccgcg aaatatgcct ggttttttga
      121 tcttgatgga acgctggcgg aaatcaaacc gcatcccgat caggtcgtcg tgcctgacaa
      181 tattctgcaa ggactacagc tactggcaac cgcaagtgat ggtgcattgg cattgatatc
      241 agggcgctca atggtggagc ttgacgcact ggcaaaacct tatcgcttcc cgttagcggg
      301 cgtgcatggg gcggagcgcc gtgacatcaa tggtaaaaca catatcgttc atctgccgga
      361 tgcgattgcg cgtgatatta gcgtgcaact gcatacagtc atcgctcagt atcccggcgc
      421 ggagctggag gcgaaaggga tggcttttgc gctgcattat cgtcaggctc cgcagcatga
      481 agacgcatta atgacattag cgcaacgtat tactcagatc tggccacaaa tggcgttaca
      541 gcagggaaag tgtgttgtcg agatcaaacc gagaggtacc agtaaaggtg aggcaattgc
      601 agcttttatg caggaagctc cctttatcgg gcgaacgccc gtatttctgg gcgatgattt
      661 aaccgatgaa tctggcttcg cagtcgttaa ccgactgggc ggaatgtcag taaaaattgg
      721 cacaggtgca actcaggcat catggcgact ggcgggtgtg ccggatgtct ggagctggct
      781 tgaaatgata accaccgcat tacaacaaaa aagagaaaat aacaggagtg atgactatga
      841 gtcgtttagt cgtagtatct aatactagag aaagaggaga aatactagat gagtcgttta
      901 gtcgtagtat ctaaccggat tgcaccacca gacgagcacg ccgccagtgc cggtggcctt
      961 gccgttggca tactgggggc actgaaagcc gcaggcggac tgtggtttgg ctggagtggt
     1021 gaaacaggga atgaggatca gccgctaaaa aaggtgaaaa aaggtaacat tacgtgggcc
     1081 tcttttaacc tcagcgaaca ggaccttgac gaatactaca accaattctc caatgccgtt
     1141 ctctggcccg cttttcatta tcggctcgat ctggtgcaat ttcagcgtcc tgcctgggac
     1201 ggctatctac gcgtaaatgc gttgctggca gataaattac tgccgctgtt gcaagacgat
     1261 gacattatct ggatccacga ttatcacctg ttgccatttg cgcatgaatt acgcaaacgg
     1321 ggagtgaata atcgcattgg tttctttctg catattcctt tcccgacacc ggaaatcttc
     1381 aacgcgctgc cgacatatga caccttgctt gaacagcttt gtgattatga tttgctgggt
     1441 ttccagacag aaaacgatcg tctggcgttc ctggattgtc tttctaacct gacccgcgtc
     1501 acgacacgta gcgcaaaaag ccatacagcc tggggcaaag catttcgaac agaagtctac
     1561 ccgatcggca ttgaaccgaa agaaatagcc aaacaggctg ccgggccact gccgccaaaa
     1621 ctggcgcaac ttaaagcgga actgaaaaac gtacaaaata tcttttctgt cgaacggctg
     1681 gattattcca aaggtttgcc agagcgtttt ctcgcctatg aagcgttgct ggaaaaatat
     1741 ccgcagcatc atggtaaaat tcgttatacc cagattgcac caacgtcgcg tggtgatgtg
     1801 caagcctatc aggatattcg tcatcagctc gaaaatgaag ctggacgaat taatggtaaa
     1861 tacgggcaat taggctggac gccgctttat tatttgaatc agcattttga ccgtaaatta
     1921 ctgatgaaaa tattccgcta ctctgacgtg ggcttagtga cgccactgcg tgacgggatg
     1981 aacctggtag caaaagagta tgttgctgct caggacccag ccaatccggg cgttcttgtt
     2041 ctttcgcaat ttgcgggagc ggcaaacgag ttaacgtcgg cgttaattgt taacccctac
     2101 gatcgtgacg aagttgcagc tgcgctggat cgtgcattga ctatgtcgct ggcggaacgt
     2161 atttcccgtc atgcagaaat gctggacgtt atcgtgaaaa acgatattaa ccactggcag
     2221 gagtgcttca ttagcgacct aaagcagata gttccgcgaa gcgcggaaag ccagcagcgc
     2281 gataaagttg ctacctttcc aaagcttgcg tagtactaga gccaggcatc aaataaaacg
     2341 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2401 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//