LOCUS       BBa_K1339017            2450 bp    DNA     linear       15-May-2024
DEFINITION  Pconst(low)-RBS-otsA-RBS-otsB-dT
ACCESSION   BBa_K1339017
VERSION     BBa_K1339017.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1486
                     /label=BBa_K1339000
     misc_feature    1499..1502
                     /label=conserved
     RBS             1495..1506
                     /label=BBa_B0034
     CDS             1513..2313
                     /label=BBa_K1339001
     stem_loop       2333..2376
                     /label=stem_loop
     misc_feature    2322..2401
                     /label=BBa_B0010
     terminator      2322..2401
                     /label=BBa_B0010
     stem_loop       2417..2436
                     /label=T7 TE
     misc_feature    2443..2443
                     /label=stop
     polyA_site      2437..2450
                     /label=polya
     misc_feature    2410..2450
                     /label=BBa_B0012
     terminator      2410..2450
                     /label=BBa_B0012
     terminator      2322..2450
                     /label=BBa_B0015
     misc_feature    44..2450
                     /label=BBa_K1339003
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaaagagg agaaatacta
       61 gatgagtcgt ttagtcgtag tatctaaccg gattgcacca ccagacgagc acgccgccag
      121 tgccggtggc cttgccgttg gcatactggg ggcactgaaa gccgcaggcg gactgtggtt
      181 tggctggagt ggtgaaacag ggaatgagga tcagccgcta aaaaaggtga aaaaaggtaa
      241 cattacgtgg gcctctttta acctcagcga acaggacctt gacgaatact acaaccaatt
      301 ctccaatgcc gttctctggc ccgcttttca ttatcggctc gatctggtgc aatttcagcg
      361 tcctgcctgg gacggctatc tacgcgtaaa tgcgttgctg gcagataaat tactgccgct
      421 gttgcaagac gatgacatta tctggatcca cgattatcac ctgttgccat ttgcgcatga
      481 attacgcaaa cggggagtga ataatcgcat tggtttcttt ctgcatattc ctttcccgac
      541 accggaaatc ttcaacgcgc tgccgacata tgacaccttg cttgaacagc tttgtgatta
      601 tgatttgctg ggtttccaga cagaaaacga tcgtctggcg ttcctggatt gtctttctaa
      661 cctgacccgc gtcacgacac gtagcgcaaa aagccataca gcctggggca aagcatttcg
      721 aacagaagtc tacccgatcg gcattgaacc gaaagaaata gccaaacagg ctgccgggcc
      781 actgccgcca aaactggcgc aacttaaagc ggaactgaaa aacgtacaaa atatcttttc
      841 tgtcgaacgg ctggattatt ccaaaggttt gccagagcgt tttctcgcct atgaagcgtt
      901 gctggaaaaa tatccgcagc atcatggtaa aattcgttat acccagattg caccaacgtc
      961 gcgtggtgat gtgcaagcct atcaggatat tcgtcatcag ctcgaaaatg aagctggacg
     1021 aattaatggt aaatacgggc aattaggctg gacgccgctt tattatttga atcagcattt
     1081 tgaccgtaaa ttactgatga aaatattccg ctactctgac gtgggcttag tgacgccact
     1141 gcgtgacggg atgaacctgg tagcaaaaga gtatgttgct gctcaggacc cagccaatcc
     1201 gggcgttctt gttctttcgc aatttgcggg agcggcaaac gagttaacgt cggcgttaat
     1261 tgttaacccc tacgatcgtg acgaagttgc agctgcgctg gatcgtgcat tgactatgtc
     1321 gctggcggaa cgtatttccc gtcatgcaga aatgctggac gttatcgtga aaaacgatat
     1381 taaccactgg caggagtgct tcattagcga cctaaagcag atagttccgc gaagcgcgga
     1441 aagccagcag cgcgataaag ttgctacctt tccaaagctt gcgtagtact agagaaagag
     1501 gagaaatact agatgacaga accgttaacc gaaacccctg aactatccgc gaaatatgcc
     1561 tggttttttg atcttgatgg aacgctggcg gaaatcaaac cgcatcccga tcaggtcgtc
     1621 gtgcctgaca atattctgca aggactacag ctactggcaa ccgcaagtga tggtgcattg
     1681 gcattgatat cagggcgctc aatggtggag cttgacgcac tggcaaaacc ttatcgcttc
     1741 ccgttagcgg gcgtgcatgg ggcggagcgc cgtgacatca atggtaaaac acatatcgtt
     1801 catctgccgg atgcgattgc gcgtgatatt agcgtgcaac tgcatacagt catcgctcag
     1861 tatcccggcg cggagctgga ggcgaaaggg atggcttttg cgctgcatta tcgtcaggct
     1921 ccgcagcatg aagacgcatt aatgacatta gcgcaacgta ttactcagat ctggccacaa
     1981 atggcgttac agcagggaaa gtgtgttgtc gagatcaaac cgagaggtac cagtaaaggt
     2041 gaggcaattg cagcttttat gcaggaagct ccctttatcg ggcgaacgcc cgtatttctg
     2101 ggcgatgatt taaccgatga atctggcttc gcagtcgtta accgactggg cggaatgtca
     2161 gtaaaaattg gcacaggtgc aactcaggca tcatggcgac tggcgggtgt gccggatgtc
     2221 tggagctggc ttgaaatgat aaccaccgca ttacaacaaa aaagagaaaa taacaggagt
     2281 gatgactatg agtcgtttag tcgtagtatc taatactaga gccaggcatc aaataaaacg
     2341 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2401 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//