BBa_K1343000
1
BBa_K1343000
P_tet->NheI->RBS->luxI->double terminator
2014-09-21T11:00:00Z
2015-05-08T01:09:59Z
P_tet: BBa_I759002- we used only the promoter, without the rest of the part (spacer (including the RBS)-cro complex) and added few bp as a spacer.
RBS: BBa_B0030.
luxI: BBa_C0061 but we removed the LVA tag and added stop codon: "tag".
Double terminator BBa_B0015
This part generates AHL (Source organism: V. fischeri).
The promoter is P_tet which induced by Tetracycline or by its analog anhydrotetracycline (aTc). Before the promoter there is the prefix site and after the promoter there is a restriction site of NheI, so the promoter can be replaced by a restriction enzyme reaction.
In addition, after the NheI restriction site, there is an RBS and luxI which is coding to AHL. At the end of the part, there is double terminator and the Suffix site.
false
false
_1718_
0
20627
9
It's complicated
true
The part has been sequenced and has been checked with a detector strain which detects AHL and produce color. We know for sure that it produces AHL.
false
Shira Attias
annotation2384874
1
Double terminator
range2384874
1
673
801
annotation2384872
1
luxI
range2384872
1
91
672
annotation2384866
1
NheI
range2384866
1
64
69
annotation2384865
1
P_tet
range2384865
1
1
63
annotation2384871
1
spacer
range2384871
1
70
90
BBa_K1343000_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacaacgctagcattaaagaggagaaaccccccatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z