LOCUS       BBa_K1343003            1147 bp    DNA     linear       12-Jun-2024
DEFINITION  plac luxR double terminator
ACCESSION   BBa_K1343003
VERSION     BBa_K1343003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    230..231
                     /label=prefix
     misc_feature    403..403
                     /label=T
     misc_feature    721..721
                     /label=A
     CDS             230..979
                     /label=luxR
     misc_feature    230..985
                     /label=BBa_C0062
     misc_feature    986..1010
                     /label=Help:Barcodes
     CDS             230..985
                     /label=BBa_C0062
     stem_loop       1030..1073
                     /label=stem_loop
     misc_feature    1019..1098
                     /label=BBa_B0010
     terminator      1019..1098
                     /label=BBa_B0010
     stem_loop       1114..1133
                     /label=T7 TE
     misc_feature    1140..1140
                     /label=stop
     polyA_site      1134..1147
                     /label=polya
     misc_feature    1107..1147
                     /label=BBa_B0012
     terminator      1107..1147
                     /label=BBa_B0012
     terminator      1019..1147
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgaaaaacat
      241 aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga
      301 tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc
      361 gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa
      421 aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta
      481 ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa
      541 aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt
      601 ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga
      661 caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc
      721 tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac
      781 caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc
      841 aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa
      901 actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga
      961 ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc
     1021 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1081 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1141 gtttata
//