| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1349000_sequence (Version 1)
|
Description
This construct encodes a truncated version of E. coli W3110 SerA ( D-3-phosphoglycerate dehydrogenase), missing the last 75 aa of the full sequence, and without stop codon. SerA is requiered for serine biosynthesis. Following the work of Peters-Wendisch et al. 2005, this mutated version of the enzyme is expected to be no-longer inhibited by Serine, and to allow the production of a high serine concentration.
Notes
The part was obtained by PCR from E.coli strain W3110 and SLIC assembly. The construction removed the EcoR1 restriction sites in the gene by the silent mutations of a GAA codon to a GAG codon.
Source
Amplified by PCR from E.coli W3110 genomic DNA.
