BBa_K1351038

BBa_K1351038 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1351038
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nikolai Peschek
Date created: 2014-10-05 11:00:00
Date modified: 2015-06-01 08:14:22

P3-promoter



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K1351038_sequence (Version 1)

    Description

    Quorum sensing dependant promotor (AIP II). Regulated by phosphorylated AgrA. Contains four AgrA binding sites upstream.

    Notes

    Several mutated illegal restriction sites. The mutated codons have been optimized regarding the codon usage of Bacillus subtilis.

    Source

    artificially synthesized

    Sequence Annotation Location Component / Role(s)
    AgrA box (binding site)
    AgrA box (binding site)
    AgrA box (binding site)
    AgrA box (binding site)
    -35
    -10
    		22,29
    44,51
    97,100
    114,121
    133,138
    160,165
    		feature/binding non_covalent_binding_site
    feature/binding non_covalent_binding_site
    non_covalent_binding_site feature/binding
    feature/binding non_covalent_binding_site
    promoter feature/promoter
    feature/promoter promoter

    igem#experience
    Works
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1351038/1
     

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