LOCUS       BBa_K1352003            2442 bp    DNA     linear       18-May-2024
DEFINITION  Ice Nucleation Protein with YFP and His-tag
ACCESSION   BBa_K1352003
VERSION     BBa_K1352003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..32
                     /label=Spacer
     misc_feature    597..625
                     /label=Spacer
     misc_feature    38..589
                     /label=Plac - LacZ
     RBS             611..614
                     /label=rbs
     misc_feature    623..623
                     /label=ATG
     misc_feature    632..1555
                     /label=INP
     misc_feature    1565..1600
                     /label=Spacer (Linker)
     misc_feature    1607..2329
                     /label=EYFP
     misc_feature    2337..2364
                     /label=Spacer
     CDS             623..2323
                     /label=INP-EYFP fusion
     misc_feature    2324..2324
                     /label=TAATAA
     promoter        309..314
                     /label=-10
     promoter        286..291
                     /label=-35
     CDS             359..589
                     /label=LacZ
     RBS             348..351
                     /label=rbs
     CDS             1..2442
                     /label=BBa_K523013
ORIGIN
        1 ttaatacgac tcactatagg gagaggaggt acgatctgct ggggcaaacc agcgtggacc
       61 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
      121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
      181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      301 ccggctcgta tgttgtgtga aattgtgagc ggataacaat ttcacacagg aaacagctat
      361 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      421 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
      541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgat actagctgat
      601 atttcgcgta aggaaatcca ttatgggatc tatgaccctg gataaagcgc tggtgctgcg
      661 cacttgtgcc aataatatgg ccgaccattg tggcctgatc tggcctgctt ctggtacagt
      721 tgaaagccgc tattggcagt ctacacgtcg tcacgaaaac gggctggttg ggctgctgtg
      781 gggtgccgga acatccgcct ttctgagcgt tcatgccgat gctcgttgga ttgtttgtga
      841 ggttgctgtt gccgacatca ttagcctgga agaaccgggt atggtgaaat ttcctcgtgc
      901 tgaagtggtc catgttggtg atcgtattag cgcctcccat ttcatttcag ctcgtcaggc
      961 tgatccagca agcacatcaa caagcacctc taccagtaca ctgacaccaa tgccaaccgc
     1021 tatccctact cctatgccgg ctgtagcctc tgttactctg cctgtggccg aacaagctcg
     1081 ccacgaagtc tttgacgttg cctctgtgtc tgctgctgct gctcctgtta acactctgcc
     1141 tgttaccacg cctcaaaatc tgcaaacggc gacctatggc tcaacactga gtggtgataa
     1201 ccattctcgt ctgattgccg ggtatggctc taacgaaacc gccggaaacc attctgatct
     1261 gatcggtggt cacgattgta cgctgatggc aggtgatcaa tctcgtctga ccgctggcaa
     1321 aaattccgtt ctgaccgctg gggcacgttc taaactgatc gggagcgaag gctctactct
     1381 gtccgccgga gaagattcta ccctgatctt tcgtctgtgg gatggtaaac gttatcgtca
     1441 gctggtcgct cgtacaggtg aaaatggtgt ggaagccgac attccgtatt atgtgaatga
     1501 ggacgatgat attgtggaca aaccggacga agatgatgat tggatcgaag tgaaaggtac
     1561 tagcagcagc attgcgagca gcagcccgag cagcgtggcg ggatctatgg tgagcaaggg
     1621 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1681 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1741 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     1801 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt
     1861 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1921 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1981 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2041 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2101 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2161 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     2221 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2281 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat actagcaaca
     2341 tatcataacg gagtgatcgc aatgggatct gttgtttgtc ggtgaacgct ctctactaga
     2401 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//