BBa_K136000
1
BBa_K136000
Promoter of fliL gene from E. coli flagella
2008-08-11T11:00:00Z
2015-05-08T01:10:02Z
This part comes from the genome of ''E.coli'' K12 strain MG1655.
''fliL'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ).
Its promoter is consitutively repressed.
It is activated by flhD<sub>4</sub>C<sub>2</sub> and by fliA with different forces ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ).
A deeper characterization is in project
true
false
_211_
0
3012
9
Discontinued
false
The primers used to amplify this sequence are :
Forward :
TCGAATTCGCGGCCGCTTCTAGAGCAAGGGCGTGTAACAGGCAAC
Reverse :
TCCTGCAGCGGCCGCTACTAGTAGTCATGTGTTGCGGTCTTCCTGTG
false
Cyprien Maisonnier
annotation1971564
1
FlhDC binding site 1
range1971564
1
44
59
annotation1971567
1
Initiation start for transcription 2
range1971567
1
127
127
annotation1971566
1
Initiation start for transcription 1
range1971566
1
116
116
annotation1971565
1
FlhDC binding site 2
range1971565
1
70
85
BBa_K136000_sequence
1
caagggcgtgtaacaggcaacagcggcgttgatattttcgcctaacgtcagaggtagcaccgtaatccgcgtcttttccccgctttgttgcgctcaagacgcaggataattagccgataagcagtagcgacacaggaagaccgcaacacatgac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z