LOCUS       BBa_K136014             2096 bp    DNA     linear       01-Jun-2024
DEFINITION  AracpBAD - gfp triparts
ACCESSION   BBa_K136014
VERSION     BBa_K136014.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..741
                     /label=GFP protein
     CDS             22..741
                     /label=BBa_E0040
     stem_loop       761..804
                     /label=stem_loop
     misc_feature    750..829
                     /label=BBa_B0010
     terminator      750..829
                     /label=BBa_B0010
     stem_loop       845..864
                     /label=T7 TE
     misc_feature    871..871
                     /label=stop
     polyA_site      865..878
                     /label=polya
     misc_feature    838..878
                     /label=BBa_B0012
     terminator      838..878
                     /label=BBa_B0012
     promoter        1966..2096
                     /label=Pbad
     promoter        1929..2000
                     /label=PC
     misc_feature    887..1965
                     /label=AraC
     promoter        887..2096
                     /label=BBa_I0500
ORIGIN
        1 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
       61 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      121 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      181 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      241 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      301 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      361 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      421 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      481 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      541 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      601 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      661 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      721 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttat gacaacttga
      901 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
      961 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
     1021 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     1081 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     1141 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     1201 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     1261 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     1321 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     1381 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     1441 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     1501 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     1561 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     1621 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     1681 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     1741 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
     1801 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
     1861 ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga
     1921 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
     1981 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
     2041 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg gctagc
//