LOCUS       BBa_K136015             2096 bp    DNA     linear       01-Jun-2024
DEFINITION  pBad/araC - GFP tripart
ACCESSION   BBa_K136015
VERSION     BBa_K136015.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1233
                     /label=RBS-1\Strong
     RBS             1226..1230
                     /label=RBS
     misc_feature    1219..1233
                     /label=BBa_B0030
     RBS             1219..1233
                     /label=BBa_B0030
     CDS             1240..1959
                     /label=GFP protein
     CDS             1240..1959
                     /label=BBa_E0040
     stem_loop       1979..2022
                     /label=stem_loop
     misc_feature    1968..2047
                     /label=BBa_B0010
     terminator      1968..2047
                     /label=BBa_B0010
     stem_loop       2063..2082
                     /label=T7 TE
     misc_feature    2089..2089
                     /label=stop
     polyA_site      2083..2096
                     /label=polya
     misc_feature    2056..2096
                     /label=BBa_B0012
     terminator      2056..2096
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt
     1261 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     1321 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     1381 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
     1441 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
     1501 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     1561 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     1621 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     1681 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     1741 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1801 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     1861 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     1921 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
     1981 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2041 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//