LOCUS       BBa_K136022              960 bp    DNA     linear       17-May-2024
DEFINITION  Weak Promoter - ECFP tripart (+LVA)
ACCESSION   BBa_K136022
VERSION     BBa_K136022.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..823
                     /label=CFP (LVA)
     misc_feature    780..817
                     /label=SsrA
     misc_feature    818..823
                     /label=2
     misc_feature    130..130
                     /label=A
     misc_feature    62..823
                     /label=BBa_E0022
     misc_feature    62..823
                     /label=BBa_E0022
     stem_loop       843..886
                     /label=stem_loop
     misc_feature    832..911
                     /label=BBa_B0010
     terminator      832..911
                     /label=BBa_B0010
     stem_loop       927..946
                     /label=T7 TE
     misc_feature    953..953
                     /label=stop
     polyA_site      947..960
                     /label=polya
     misc_feature    920..960
                     /label=BBa_B0012
     terminator      920..960
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      121 cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      241 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
      301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      481 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
      541 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      661 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      721 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagag
      781 gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga gccaggcatc
      841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      901 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//