LOCUS       BBa_K136025              938 bp    DNA     linear       01-Jun-2024
DEFINITION  gfp generator -TetR promotor
ACCESSION   BBa_K136025
VERSION     BBa_K136025.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..739
                     /label=GFP protein
     CDS             20..739
                     /label=BBa_E0040
     stem_loop       759..802
                     /label=stem_loop
     misc_feature    748..827
                     /label=BBa_B0010
     terminator      748..827
                     /label=BBa_B0010
     stem_loop       843..862
                     /label=T7 TE
     misc_feature    869..869
                     /label=stop
     polyA_site      863..876
                     /label=polya
     misc_feature    836..876
                     /label=BBa_B0012
     terminator      836..876
                     /label=BBa_B0012
     misc_feature    885..903
                     /label=TetR 1
     misc_feature    885..938
                     /label=BBa_R0040
     promoter        904..909
                     /label=-35
     misc_feature    910..928
                     /label=TetR 2
     promoter        927..932
                     /label=-10
     promoter        885..938
                     /label=BBa_R0040
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      781 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      841 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag
      901 agattgacat ccctatcagt gatagagata ctgagcac
//
LOCUS       BBa_K136047              938 bp    DNA     linear       01-Jun-2024
DEFINITION  gfp generator - tetR promoter
ACCESSION   BBa_K136047
VERSION     BBa_K136047.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..739
                     /label=GFP protein
     CDS             20..739
                     /label=BBa_E0040
     stem_loop       759..802
                     /label=stem_loop
     misc_feature    748..827
                     /label=BBa_B0010
     terminator      748..827
                     /label=BBa_B0010
     stem_loop       843..862
                     /label=T7 TE
     misc_feature    869..869
                     /label=stop
     polyA_site      863..876
                     /label=polya
     misc_feature    836..876
                     /label=BBa_B0012
     terminator      836..876
                     /label=BBa_B0012
     misc_feature    885..903
                     /label=TetR 1
     misc_feature    885..938
                     /label=BBa_R0040
     promoter        904..909
                     /label=-35
     misc_feature    910..928
                     /label=TetR 2
     promoter        927..932
                     /label=-10
     promoter        885..938
                     /label=BBa_R0040
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      781 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      841 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag
      901 agattgacat ccctatcagt gatagagata ctgagcac
//