LOCUS       BBa_K136033             1610 bp    DNA     linear       13-May-2024
DEFINITION  RBS lasI - gfp tripart
ACCESSION   BBa_K136033
VERSION     BBa_K136033.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..780
                     /label=CFP (LVA)
     misc_feature    737..774
                     /label=SsrA
     misc_feature    775..780
                     /label=2
     misc_feature    87..87
                     /label=A
     misc_feature    19..780
                     /label=BBa_E0022
     misc_feature    19..780
                     /label=BBa_E0022
     stem_loop       800..843
                     /label=stem_loop
     misc_feature    789..868
                     /label=BBa_B0010
     terminator      789..868
                     /label=BBa_B0010
     stem_loop       884..903
                     /label=T7 TE
     misc_feature    910..910
                     /label=stop
     polyA_site      904..917
                     /label=polya
     misc_feature    877..917
                     /label=BBa_B0012
     terminator      877..917
                     /label=BBa_B0012
     misc_feature    930..933
                     /label=conserved
     RBS             926..937
                     /label=BBa_B0034
     CDS             944..1546
                     /label=LasI
     misc_feature    1547..1579
                     /label=LVA
     misc_feature    1586..1610
                     /label=Help:Barcodes
     CDS             944..1585
                     /label=BBa_C0078
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc
      241 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc
      481 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
      781 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttatatac tagagaaaga ggagaaatac tagatgatcg ttcagatcgg
      961 tcgtcgtgaa gagttcgaca aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt
     1021 tttcaaagaa cgtaaaggtt gggacgtttc cgttatcgac gaaatggaaa tcgacggtta
     1081 cgacgctctg tccccgtact acatgctgat ccaggaagac accccggaag ctcaggtttt
     1141 cggttgctgg cgtatcttcg acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga
     1201 actgctgcac ggtaaagaag ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc
     1261 tatcaactcc ggtcagaaag gttccctggg tttctccgac tgcaccctgg aagctatgcg
     1321 tgctctggct cgttactcct tgcagaacga catccagacc ctggttaccg ttaccaccgt
     1381 tggtgttgaa aaaatgatga tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct
     1441 gaaaatcggt atcgaacgtg ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat
     1501 cgctctgtac ggtggtgttc tggttgaaca gcgtctggct gtttccgctg ctaacgacga
     1561 aaactacgct ctggttgctt aataactctg atagtgctag tgtagatctc
//