LOCUS       BBa_K136034             1571 bp    DNA     linear       14-May-2024
DEFINITION  RBS lasI - gfp tripart
ACCESSION   BBa_K136034
VERSION     BBa_K136034.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..621
                     /label=LasI
     misc_feature    622..654
                     /label=LVA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             19..660
                     /label=BBa_C0078
     RBS             694..708
                     /label=RBS-1\Strong
     RBS             701..705
                     /label=RBS
     misc_feature    694..708
                     /label=BBa_B0030
     RBS             694..708
                     /label=BBa_B0030
     CDS             715..1434
                     /label=GFP protein
     CDS             715..1434
                     /label=BBa_E0040
     stem_loop       1454..1497
                     /label=stem_loop
     misc_feature    1443..1522
                     /label=BBa_B0010
     terminator      1443..1522
                     /label=BBa_B0010
     stem_loop       1538..1557
                     /label=T7 TE
     misc_feature    1564..1564
                     /label=stop
     polyA_site      1558..1571
                     /label=polya
     misc_feature    1531..1571
                     /label=BBa_B0012
     terminator      1531..1571
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gatcgttcag atcggtcgtc gtgaagagtt cgacaaaaaa
       61 ctgctgggtg aaatgcacaa actgcgtgct caggttttca aagaacgtaa aggttgggac
      121 gtttccgtta tcgacgaaat ggaaatcgac ggttacgacg ctctgtcccc gtactacatg
      181 ctgatccagg aagacacccc ggaagctcag gttttcggtt gctggcgtat cttcgacacc
      241 accggtccgt acatgctgaa aaacaccttc ccggaactgc tgcacggtaa agaagctccg
      301 tgctccccgc acatctggga actgtcccgt ttcgctatca actccggtca gaaaggttcc
      361 ctgggtttct ccgactgcac cctggaagct atgcgtgctc tggctcgtta ctccttgcag
      421 aacgacatcc agaccctggt taccgttacc accgttggtg ttgaaaaaat gatgatccgt
      481 gctggtctgg acgtttcccg tttcggtccg cacctgaaaa tcggtatcga acgtgctgtt
      541 gctctgcgta tcgaactgaa cgctaaaacc cagatcgctc tgtacggtgg tgttctggtt
      601 gaacagcgtc tggctgtttc cgctgctaac gacgaaaact acgctctggt tgcttaataa
      661 ctctgatagt gctagtgtag atctctacta gagattaaag aggagaaata ctagatgcgt
      721 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      781 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      841 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      901 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      961 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1021 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1081 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1141 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1201 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1261 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1321 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1381 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1441 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1501 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1561 ctgcgtttat a
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