LOCUS       BBa_K136056             1610 bp    DNA     linear       14-May-2024
DEFINITION  RBS - lasI - ECFP Tripart (+LVA)
ACCESSION   BBa_K136056
VERSION     BBa_K136056.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..621
                     /label=LasI
     misc_feature    622..654
                     /label=LVA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             19..660
                     /label=BBa_C0078
     misc_feature    1..685
                     /label=BBa_S03154
     misc_feature    698..701
                     /label=conserved
     RBS             694..705
                     /label=BBa_B0034
     CDS             712..1473
                     /label=CFP (LVA)
     misc_feature    1430..1467
                     /label=SsrA
     misc_feature    1468..1473
                     /label=2
     misc_feature    780..780
                     /label=A
     misc_feature    712..1473
                     /label=BBa_E0022
     misc_feature    712..1473
                     /label=BBa_E0022
     stem_loop       1493..1536
                     /label=stem_loop
     misc_feature    1482..1561
                     /label=BBa_B0010
     terminator      1482..1561
                     /label=BBa_B0010
     stem_loop       1577..1596
                     /label=T7 TE
     misc_feature    1603..1603
                     /label=stop
     polyA_site      1597..1610
                     /label=polya
     misc_feature    1570..1610
                     /label=BBa_B0012
     terminator      1570..1610
                     /label=BBa_B0012
     misc_feature    694..1610
                     /label=BBa_E0422
ORIGIN
        1 aaagaggaga aatactagat gatcgttcag atcggtcgtc gtgaagagtt cgacaaaaaa
       61 ctgctgggtg aaatgcacaa actgcgtgct caggttttca aagaacgtaa aggttgggac
      121 gtttccgtta tcgacgaaat ggaaatcgac ggttacgacg ctctgtcccc gtactacatg
      181 ctgatccagg aagacacccc ggaagctcag gttttcggtt gctggcgtat cttcgacacc
      241 accggtccgt acatgctgaa aaacaccttc ccggaactgc tgcacggtaa agaagctccg
      301 tgctccccgc acatctggga actgtcccgt ttcgctatca actccggtca gaaaggttcc
      361 ctgggtttct ccgactgcac cctggaagct atgcgtgctc tggctcgtta ctccttgcag
      421 aacgacatcc agaccctggt taccgttacc accgttggtg ttgaaaaaat gatgatccgt
      481 gctggtctgg acgtttcccg tttcggtccg cacctgaaaa tcggtatcga acgtgctgtt
      541 gctctgcgta tcgaactgaa cgctaaaacc cagatcgctc tgtacggtgg tgttctggtt
      601 gaacagcgtc tggctgtttc cgctgctaac gacgaaaact acgctctggt tgcttaataa
      661 ctctgatagt gctagtgtag atctctacta gagaaagagg agaaatacta gatggtgagc
      721 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgtg
      781 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      841 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      901 accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      961 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1021 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1081 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1141 tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     1201 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1261 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1321 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1381 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagag gcctgctgca
     1441 aacgacgaaa actacgcttt agtagcttaa taatactaga gccaggcatc aaataaaacg
     1501 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1561 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//