LOCUS       BBa_K1361001            1548 bp    DNA     linear       19-May-2024
DEFINITION  Curli Fiber generator under the control of T7 promoter with a 
            relatively weak expression of CsgA,C
ACCESSION   BBa_K1361001
VERSION     BBa_K1361001.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..41
                     /label=start
     misc_feature    492..494
                     /label=stop
     CDS             39..494
                     /label=CsgB
     CDS             39..494
                     /label=BBa_K1361997
     stem_loop       514..557
                     /label=stem_loop
     misc_feature    503..582
                     /label=BBa_B0010
     terminator      503..582
                     /label=BBa_B0010
     stem_loop       598..617
                     /label=T7 TE
     misc_feature    624..624
                     /label=stop
     polyA_site      618..631
                     /label=polya
     misc_feature    591..631
                     /label=BBa_B0012
     terminator      591..631
                     /label=BBa_B0012
     terminator      503..631
                     /label=BBa_B0015
     promoter        640..674
                     /label=BBa_J23104
     RBS             683..695
                     /label=RBS-3\Weak
     RBS             689..692
                     /label=RBS
     misc_feature    683..695
                     /label=BBa_B0032
     RBS             683..695
                     /label=BBa_B0032
     misc_feature    640..695
                     /label=BBa_K608003
     CDS             702..1157
                     /label=CsgA
     misc_feature    702..704
                     /label=start
     misc_feature    1155..1157
                     /label=stop
     CDS             1216..1548
                     /label=CsgC
     misc_feature    1216..1218
                     /label=start
     misc_feature    1546..1548
                     /label=stop
     misc_feature    702..1548
                     /label=BBa_K1361998
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaaaaacaaa ttgttattta
       61 tgatgttaac aatactgggt gcgcctggga ttgcagccgc agcaggttat gatttagcta
      121 attcagaata taacttcgcg gtaaatgaat tgagtaagtc ttcatttaat caggcagcca
      181 taattggtca agctgggact aataatagtg ctcagttacg gcagggaggc tcaaaacttt
      241 tggcggttgt tgcgcaagaa ggtagtagca accgggcaaa gattgaccag acaggagatt
      301 ataaccttgc atatattgat caggcgggca gtgccaacga tgccagtatt tcgcaaggtg
      361 cttatggtaa tactgcgatg attatccaga aaggttctgg taataaagca aatattacac
      421 agtatggtac tcaaaaaacg gcaattgtag tgcagagaca gtcgcaaatg gctattcgcg
      481 tgacacaacg ttaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      541 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      601 tcaccttcgg gtgggccttt ctgcgtttat atactagagt tgacagctag ctcagtccta
      661 ggtattgtgc tagctactag agtcacacag gaaagtacta gatgaaactt ttaaaagtag
      721 cagcaattgc agcaatcgta ttctccggta gcgctctggc aggtgttgtt cctcagtacg
      781 gcggcggcgg taaccacggt ggtggcggta ataatagcgg cccaaattct gagctgaaca
      841 tttaccagta cggtggcggt aactctgcac ttgctctgca aactgatgcc cgtaactctg
      901 acttgactat tacccagcat ggcggcggta atggtgcaga tgttggtcag ggctcagatg
      961 acagctcaat cgatctgacc caacgtggct tcggtaacag cgctactctt gatcagtgga
     1021 acggcaaaaa ttctgaaatg acggttaaac agttcggtgg tggcaacggt gccgcagttg
     1081 accagactgc atctaactcc tccgtcaacg tgactcaggt tggctttggt aacaacgcga
     1141 ccgctcatca gtactaatac atcatttgta ttacagaaac agggcgcaag ccctgttttt
     1201 tttcgggaga agaatatgaa tacgttatta ctccttgcgg cactttccag tcagataacc
     1261 tttaatacga cccagcaagg ggatgtgtat accattattc ctgaagtcac tcttactcaa
     1321 tcttgtctgt gcagagtaca aatattgtcc ctgcgcgaag gcagttcagg gcaaagtcag
     1381 acgaagcaag aaaagaccct ttcattgcct gctaatcaac ccattgcttt gacgaagttg
     1441 agtttaaata tttccccgga cgatcgggtg aaaatagttg ttactgtttc tgatggacag
     1501 tcacttcatt tatcacaaca atggccgccc tcttcagaaa agtcttaa
//