BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_J23104
1
BBa_J23104
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
isolated from library of promoters
replace later
false
false
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1361995
1
BBa_K1361995
CsgBtrunc, curli fiber nucleator with a C-terminnal deletion
2014-10-03T11:00:00Z
2015-05-08T01:10:04Z
This part is collected by PCR from genome of E coli. DH5alpha.
This part is Curli fiber nucleator subunit protein CsgB with deletion of C-terminal amino acid from 133 to 155 that is lost the ability to attach outer membrane of the cell.
false
false
_1737_
0
18617
9
Not in stock
false
Deletion is designed referring Hammer, Neal D., Jens C. Schmidt, and Matthew R. Chapman. "The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization." Proceedings of the National Academy of Sciences 104.30 (2007): 12494-12499.
false
Shoujie Sun
annotation2393388
1
cds
range2393388
1
1
399
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K1361998
1
BBa_K1361998
Curli Fiber assembly associated protein CsgA, CsgC
2014-10-02T11:00:00Z
2015-05-08T01:10:04Z
PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.
CsgA, CsgC genes are directly PCRed from E coli. DH5alpha genome as a whole. There is a scar sequence between CsgA and CsgC coding region that contains a promoter sequence may constitutively transcript CsgC gene.
CsgA protein is the major subunit of Curli Fiber. CsgC may regulate CsgG outer membrane assembly of channel protein CsgG and pore activity through modification of C230 in CsgG.
false
false
_1737_
0
16915
9
Not in stock
false
The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
false
Shoujie Sun
annotation2392516
1
start
range2392516
1
515
517
annotation2392514
1
stop
range2392514
1
454
456
annotation2392515
1
CsgC
range2392515
1
515
847
annotation2392517
1
stop
range2392517
1
845
847
annotation2392512
1
CsgA
range2392512
1
1
456
annotation2392513
1
start
range2392513
1
1
3
BBa_K1361002
1
BBa_K1361002
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
2014-10-03T11:00:00Z
2015-05-08T01:10:03Z
CsgAC and CsgBtrunc were collected by PCR from genome of E coli DH5alpha respectively.
This part contains the major subunit of curli fiber CsgA and its nucleator CsgBtrunc, in which CsgA is constitutive expression whereas CsgBtrunc is under the control of T7 promotor.
This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
false
false
_1737_
0
18617
9
It's complicated
false
The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
false
Shoujie Sun
component2398541
1
BBa_B0015
component2398532
1
BBa_B0034
component2398534
1
BBa_K1361995
component2398553
1
BBa_K1361998
component2398546
1
BBa_K608003
component2398530
1
BBa_K206000
annotation2398532
1
BBa_B0034
range2398532
1
139
150
annotation2398553
1
BBa_K1361998
range2398553
1
763
1609
annotation2398534
1
BBa_K1361995
range2398534
1
157
555
annotation2398530
1
BBa_K206000
range2398530
1
1
130
annotation2398541
1
BBa_B0015
range2398541
1
564
692
annotation2398546
1
BBa_K608003
range2398546
1
701
756
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K608003
1
BBa_K608003
strong Promoter , medium RBS
2011-09-14T11:00:00Z
2015-05-08T01:12:52Z
composite
Released HQ 2013
strong Promotor , medium RBS
false
false
_780_
0
9115
9
In stock
false
composite
false
Julia M??ller
component2128651
1
BBa_B0032
component2128649
1
BBa_J23104
annotation2128651
1
BBa_B0032
range2128651
1
44
56
annotation2128649
1
BBa_J23104
range2128649
1
1
35
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K206000
1
pBAD
pBAD strong
2009-10-13T11:00:00Z
2015-05-08T01:11:23Z
The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
Released HQ 2013
pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
false
false
_307_
0
4172
9
In stock
true
There were no special design considerations.
false
Amelia Hardjasa
annotation2049254
1
AraI2
range2049254
1
61
78
annotation2049252
1
promoter
range2049252
1
1
131
annotation2049253
1
AraI1
range2049253
1
40
57
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1361002_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
BBa_K608003_sequence
1
ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaag
BBa_K1361998_sequence
1
atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1361995_sequence
1
atgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_J23104_sequence
1
ttgacagctagctcagtcctaggtattgtgctagc
BBa_K206000_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z