LOCUS       BBa_K1362002            1192 bp    DNA     linear       12-May-2024
DEFINITION  Sortase A circularization construct (with His6)
ACCESSION   BBa_K1362002
VERSION     BBa_K1362002.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    36..47
                     /label=BBa_K1362445
     misc_feature    48..68
                     /label=BBa_K1362452
     misc_feature    69..74
                     /label=BBa_K1362427
     CDS             75..162
                     /label=end of LacI coding region (inactive)
     misc_feature    75..274
                     /label=BBa_R0010
     misc_feature    163..200
                     /label=CAP binding site
     promoter        211..216
                     /label=-35
     promoter        235..240
                     /label=-10
     misc_feature    240..274
                     /label=LacI binding site
     misc_feature    247..247
                     /label=start
     promoter        75..274
                     /label=BBa_R0010
     misc_feature    287..290
                     /label=conserved
     RBS             283..294
                     /label=BBa_B0034
     CDS             301..975
                     /label=mrfp1
     misc_feature    982..1006
                     /label=Help:Barcodes
     CDS             301..981
                     /label=BBa_E1010
     stem_loop       1026..1069
                     /label=stem_loop
     misc_feature    1015..1094
                     /label=BBa_B0010
     terminator      1015..1094
                     /label=BBa_B0010
     stem_loop       1110..1129
                     /label=T7 TE
     misc_feature    1136..1136
                     /label=stop
     polyA_site      1130..1143
                     /label=polya
     misc_feature    1103..1143
                     /label=BBa_B0012
     terminator      1103..1143
                     /label=BBa_B0012
     misc_feature    75..1143
                     /label=BBa_J04450
     misc_feature    1144..1150
                     /label=BBa_K1362424
     misc_feature    1151..1168
                     /label=BBa_K1362454
     misc_feature    1169..1186
                     /label=BBa_K1362457
     misc_feature    1187..1191
                     /label=stop
     misc_feature    1187..1192
                     /label=BBa_J70594
     misc_feature    36..1192
                     /label=BBa_K1362202
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatggg cagtagtgag aatctttact
       61 tccagggttg agaccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      121 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      181 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      241 gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga gaaatactag
      301 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
      361 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      421 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      481 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      541 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      601 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      661 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      721 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      781 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      841 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      901 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      961 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
     1021 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1081 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1141 ataggtctca cttcctgaaa ctggtggtca tcaccatcac catcactaat aa
//