LOCUS       BBa_K1362003            1477 bp    DNA     linear       12-May-2024
DEFINITION  Sortase A circularization construct (with Smt3 and His6)
ACCESSION   BBa_K1362003
VERSION     BBa_K1362003.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    36..38
                     /label=start codon
     misc_feature    36..38
                     /label=BBa_K371056
     misc_feature    39..332
                     /label=Smt3
     misc_feature    39..332
                     /label=BBa_K1362429
     misc_feature    333..353
                     /label=BBa_K1362453
     misc_feature    354..359
                     /label=BBa_K1362427
     CDS             360..447
                     /label=end of LacI coding region (inactive)
     misc_feature    360..559
                     /label=BBa_R0010
     misc_feature    448..485
                     /label=CAP binding site
     promoter        496..501
                     /label=-35
     promoter        520..525
                     /label=-10
     misc_feature    525..559
                     /label=LacI binding site
     misc_feature    532..532
                     /label=start
     promoter        360..559
                     /label=BBa_R0010
     misc_feature    572..575
                     /label=conserved
     RBS             568..579
                     /label=BBa_B0034
     CDS             586..1260
                     /label=mrfp1
     misc_feature    1267..1291
                     /label=Help:Barcodes
     CDS             586..1266
                     /label=BBa_E1010
     stem_loop       1311..1354
                     /label=stem_loop
     misc_feature    1300..1379
                     /label=BBa_B0010
     terminator      1300..1379
                     /label=BBa_B0010
     stem_loop       1395..1414
                     /label=T7 TE
     misc_feature    1421..1421
                     /label=stop
     polyA_site      1415..1428
                     /label=polya
     misc_feature    1388..1428
                     /label=BBa_B0012
     terminator      1388..1428
                     /label=BBa_B0012
     misc_feature    360..1428
                     /label=BBa_J04450
     misc_feature    1429..1435
                     /label=BBa_K1362424
     misc_feature    1436..1453
                     /label=BBa_K1362455
     misc_feature    1454..1471
                     /label=BBa_K1362457
     misc_feature    1472..1476
                     /label=stop
     misc_feature    1472..1477
                     /label=BBa_J70594
     misc_feature    36..1477
                     /label=BBa_K1362203
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgtc ggactcagaa gtcaatcaag
       61 aagctaagcc agaggtcaag ccagaagtca agcctgagac tcacatcaat ttaaaggtgt
      121 ccgatggatc ttcagagatc ttcttcaaga tcaaaaagac cactccttta agaaggctga
      181 tggaagcgtt cgctaaaaga cagggtaagg aaatggactc cttaagattc ttgtacgacg
      241 gtattagaat ccaagctgat cagacccctg aagatttgga catggaggat aacgatatta
      301 ttgaggctca cagagaacag attggtggat ccgaaaacct gtacttccag ggttgagacc
      361 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      421 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      481 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      541 cggataacaa tttcacacat actagagaaa gaggagaaat actagatggc ttcctccgaa
      601 gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac
      661 gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa
      721 ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc
      781 cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg
      841 tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt
      901 accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt
      961 ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct
     1021 tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg
     1081 aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa
     1141 ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac
     1201 gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct
     1261 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     1321 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1381 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatagg tctcacttcc
     1441 ggaaaccggt ggtcatcacc atcaccatca ctaataa
//