LOCUS       BBa_K1362004            1195 bp    DNA     linear       12-May-2024
DEFINITION  Sortase A non-circularization construct (with His6)
ACCESSION   BBa_K1362004
VERSION     BBa_K1362004.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    36..47
                     /label=BBa_K1362445
     misc_feature    48..65
                     /label=BBa_K1362458
     misc_feature    66..74
                     /label=BBa_K1362449
     misc_feature    75..95
                     /label=BBa_K1362453
     misc_feature    96..101
                     /label=BBa_K1362447
     CDS             102..189
                     /label=end of LacI coding region (inactive)
     misc_feature    102..301
                     /label=BBa_R0010
     misc_feature    190..227
                     /label=CAP binding site
     promoter        238..243
                     /label=-35
     promoter        262..267
                     /label=-10
     misc_feature    267..301
                     /label=LacI binding site
     misc_feature    274..274
                     /label=start
     promoter        102..301
                     /label=BBa_R0010
     misc_feature    314..317
                     /label=conserved
     RBS             310..321
                     /label=BBa_B0034
     CDS             328..1002
                     /label=mrfp1
     misc_feature    1009..1033
                     /label=Help:Barcodes
     CDS             328..1008
                     /label=BBa_E1010
     stem_loop       1053..1096
                     /label=stem_loop
     misc_feature    1042..1121
                     /label=BBa_B0010
     terminator      1042..1121
                     /label=BBa_B0010
     stem_loop       1137..1156
                     /label=T7 TE
     misc_feature    1163..1163
                     /label=stop
     polyA_site      1157..1170
                     /label=polya
     misc_feature    1130..1170
                     /label=BBa_B0012
     terminator      1130..1170
                     /label=BBa_B0012
     misc_feature    102..1170
                     /label=BBa_J04450
     misc_feature    1171..1177
                     /label=BBa_K1362424
     misc_feature    1178..1189
                     /label=BBa_K1362459
     misc_feature    1190..1194
                     /label=stop
     misc_feature    1190..1195
                     /label=BBa_J70594
     misc_feature    36..1195
                     /label=BBa_K1362204
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatggg cagtagtcat catcatcatc
       61 atcacagcag cggcgaaaac ctgtacttcc agggtagaga ccaatacgca aaccgcctct
      121 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      181 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      241 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      301 atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc
      361 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt
      421 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt
      481 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct
      541 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc
      601 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc
      661 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc
      721 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac
      781 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt
      841 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt
      901 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
      961 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc
     1021 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1081 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1141 caccttcggg tgggcctttc tgcgtttata ggtctcactt ccggaaacct aataa
//