LOCUS       BBa_K1362005            1522 bp    DNA     linear       12-May-2024
DEFINITION  Sortase A non-circularization construct (with Smt3 and His6)
ACCESSION   BBa_K1362005
VERSION     BBa_K1362005.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    36..47
                     /label=BBa_K1362446
     misc_feature    48..65
                     /label=BBa_K1362458
     misc_feature    66..74
                     /label=BBa_K1362449
     misc_feature    75..92
                     /label=BBa_K1362456
     misc_feature    93..107
                     /label=BBa_K1362448
     misc_feature    108..401
                     /label=Smt3
     misc_feature    108..401
                     /label=BBa_K1362429
     misc_feature    402..422
                     /label=BBa_K1362453
     misc_feature    423..428
                     /label=BBa_K1362447
     CDS             429..516
                     /label=end of LacI coding region (inactive)
     misc_feature    429..628
                     /label=BBa_R0010
     misc_feature    517..554
                     /label=CAP binding site
     promoter        565..570
                     /label=-35
     promoter        589..594
                     /label=-10
     misc_feature    594..628
                     /label=LacI binding site
     misc_feature    601..601
                     /label=start
     promoter        429..628
                     /label=BBa_R0010
     misc_feature    641..644
                     /label=conserved
     RBS             637..648
                     /label=BBa_B0034
     CDS             655..1329
                     /label=mrfp1
     misc_feature    1336..1360
                     /label=Help:Barcodes
     CDS             655..1335
                     /label=BBa_E1010
     stem_loop       1380..1423
                     /label=stem_loop
     misc_feature    1369..1448
                     /label=BBa_B0010
     terminator      1369..1448
                     /label=BBa_B0010
     stem_loop       1464..1483
                     /label=T7 TE
     misc_feature    1490..1490
                     /label=stop
     polyA_site      1484..1497
                     /label=polya
     misc_feature    1457..1497
                     /label=BBa_B0012
     terminator      1457..1497
                     /label=BBa_B0012
     misc_feature    429..1497
                     /label=BBa_J04450
     misc_feature    1498..1504
                     /label=BBa_K1362424
     misc_feature    1505..1516
                     /label=BBa_K1362459
     misc_feature    1517..1521
                     /label=stop
     misc_feature    1517..1522
                     /label=BBa_J70594
     misc_feature    36..1522
                     /label=BBa_K1362205
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcatgtcg gactcagaag
      121 tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact cacatcaatt
      181 taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc actcctttaa
      241 gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc ttaagattct
      301 tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac atggaggata
      361 acgatattat tgaggctcac agagaacaga ttggtggatc cgaaaacctg tacttccagg
      421 gtagagacca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      481 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      541 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      601 aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaata ctagatggct
      661 tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt
      721 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag
      781 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc
      841 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac
      901 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt
      961 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt
     1021 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt
     1081 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
     1141 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
     1201 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
     1261 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
     1321 accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa
     1381 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1441 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttataggt
     1501 ctcacttccg gaaacctaat aa
//