LOCUS       BBa_K1362012             998 bp    DNA     linear       29-Apr-2024
DEFINITION  RBS + Circular λ-Lysozyme with His6 linker
ACCESSION   BBa_K1362012
VERSION     BBa_K1362012.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..139
                     /label=BBa_K1362401
     misc_feature    140..143
                     /label=BBa_K1362419
     misc_feature    144..152
                     /label=CWE
     misc_feature    144..152
                     /label=BBa_K1362433
     CDS             153..623
                     /label=LaLy
     misc_feature    153..623
                     /label=BBa_K1362432
     misc_feature    624..653
                     /label=H6-linker
     misc_feature    624..653
                     /label=BBa_K1362435
     misc_feature    654..662
                     /label=RGK
     misc_feature    654..662
                     /label=BBa_K1362434
     misc_feature    663..666
                     /label=BBa_K1362415
     misc_feature    667..968
                     /label=BBa_K1362400
     misc_feature    969..972
                     /label=stop
     misc_feature    969..972
                     /label=BBa_K1362421
     misc_feature    996..998
                     /label=stop
     misc_feature    993..995
                     /label=stop
     misc_feature    975..992
                     /label=iGEMHD
     misc_feature    973..998
                     /label=BBa_K1362999
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgat caaaatagcc acacgtaaat
       61 atttaggcaa acaaaatgtc tatgacattg gagttgagcg cgaccataat tttgcactca
      121 aaaatggctt catagcttcc aactgctggg aagtagaaat caataatcaa cgtaaggcgt
      181 tcctcgatat gctggcgtgg tcggagggaa ctgataacgg acgtcagaaa accagaaatc
      241 atggttatga cgtcattgta ggcggagagc tatttactga ttactccgat caccctcgca
      301 aacttgtcac gctaaaccca aaactcaaat caacaggcgc cggacgctac cagcttcttt
      361 cccgttggtg ggatgcctac cgcaagcagc ttggcctgaa agacttctct ccgaaaagtc
      421 aggacgctgt ggcattgcag cagattaagg agcgtggcgc tttacctatg attgatcgtg
      481 gtgatatccg tcaggcaatc gaccgttgca gcaatatctg ggcttcactg ccgggcgctg
      541 gttatggtca gttcgagcat aaggctgaca gcctgattgc aaaattcaaa gaagcgggcg
      601 gaacggtcag agagattgat gtaggcagcc atcatcatca tcatcacagc ggccgtggta
      661 aatgcttaag ctatgaaacg gaaatattga cagtagaata tggattatta ccgattggta
      721 aaattgtaga aaagcgcatc gaatgtactg tttatagcgt tgataataat ggaaatattt
      781 atacacaacc tgtagcacaa tggcacgatc gcggagaaca agaggtgttt gagtattgtt
      841 tggaagatgg ttcattgatt cgggcaacaa aagaccataa gtttatgact gttgatggtc
      901 aaatgttgcc aattgatgaa atatttgaac gtgaattgga tttgatgcgg gttgataatt
      961 tgccgaatta ataaatcggt gaaatgcacg actgatag
//