LOCUS       BBa_K1362021            1070 bp    DNA     linear       01-May-2024
DEFINITION  RBS + Circular Xylanase with His6 linker
ACCESSION   BBa_K1362021
VERSION     BBa_K1362021.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..139
                     /label=BBa_K1362401
     misc_feature    140..143
                     /label=BBa_K1362419
     misc_feature    144..152
                     /label=CWE
     misc_feature    144..152
                     /label=BBa_K1362433
     CDS             153..707
                     /label=Xylanase
     misc_feature    153..707
                     /label=BBa_K1362441
     misc_feature    708..723
                     /label=His6
     misc_feature    708..725
                     /label=BBa_K1362443
     misc_feature    726..734
                     /label=RGK
     misc_feature    726..734
                     /label=BBa_K1362434
     misc_feature    735..738
                     /label=BBa_K1362415
     misc_feature    739..1040
                     /label=BBa_K1362400
     misc_feature    1041..1044
                     /label=stop
     misc_feature    1041..1044
                     /label=BBa_K1362421
     misc_feature    1068..1070
                     /label=stop
     misc_feature    1065..1067
                     /label=stop
     misc_feature    1047..1064
                     /label=iGEMHD
     misc_feature    1045..1070
                     /label=BBa_K1362999
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgat caaaatagcc acacgtaaat
       61 atttaggcaa acaaaatgtc tatgacattg gagttgagcg cgaccataat tttgcactca
      121 aaaatggctt catagcttcc aactgctggg aagctagcac agactactgg caaaattgga
      181 ctgatggggg cggtatagta aacgctgtca atgggtctgg cgggaattac agtgttaatt
      241 ggtctaatac cggaaatttt gttgttggta aaggttggac tacaggttcg ccatttagga
      301 cgataaacta taatgccgga gtttgggcgc cgaatggcaa tggatattta actttatatg
      361 gttggacgag atcacctctc atagaatatt atgtagtgga ttcatggggt acttatagac
      421 ctactggaac gtataaaggt actgtaaaaa gtgatggggg tacatatgac atatatacaa
      481 ctacacgtta taacgcacct tccattgatg gcgatcgcac tacttttacg cagtactgga
      541 gtgttcgcca gtcgaagaga ccaaccggaa gcaacgctac aatcactttc agcaatcatg
      601 tgaacgcatg gaagagccat ggaatgaatc tgggcagtaa ttgggcttac caagtcatgg
      661 cgacagaagg atatcaaagt agtggaagtt ctaacgtaac agtgtggcat caccatcacc
      721 atcatcgtgg taaatgctta agctatgaaa cggaaatatt gacagtagaa tatggattat
      781 taccgattgg taaaattgta gaaaagcgca tcgaatgtac tgtttatagc gttgataata
      841 atggaaatat ttatacacaa cctgtagcac aatggcacga tcgcggagaa caagaggtgt
      901 ttgagtattg tttggaagat ggttcattga ttcgggcaac aaaagaccat aagtttatga
      961 ctgttgatgg tcaaatgttg ccaattgatg aaatatttga acgtgaattg gatttgatgc
     1021 gggttgataa tttgccgaat taataaatcg gtgaaatgca cgactgatag
//