BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1362023
1
BBa_K1362023
RBS + Xylanase (with His6)
2014-10-07T11:00:00Z
2015-05-08T01:10:04Z
???
???
false
false
_1738_
0
22920
9
In stock
false
???
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
component2409641
1
BBa_K1362441
component2409645
1
BBa_J70594
component2409638
1
BBa_G0000
component2409637
1
BBa_B0034
component2409639
1
BBa_K1362414
component2409643
1
BBa_K1362442
annotation2409637
1
BBa_B0034
range2409637
1
1
12
annotation2409641
1
BBa_K1362441
range2409641
1
22
576
annotation2409643
1
BBa_K1362442
range2409643
1
577
606
annotation2409639
1
BBa_K1362414
range2409639
1
19
21
annotation2409638
1
BBa_G0000
range2409638
1
13
18
annotation2409645
1
BBa_J70594
range2409645
1
607
612
BBa_K1362414
1
RFC105 A
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[http://2014.igem.org/Team:Heidelberg|wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362442
1
H6
His6 tag with spacers
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
???
???
false
false
_1738_
0
22920
9
Not in stock
false
???
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2407316
1
His6
range2407316
1
1
30
BBa_J70594
1
BBa_J70594
RFC12 TAATAA Tail Domain
2010-06-17T11:00:00Z
2015-05-08T01:08:25Z
Common Knowledge
A RFC12 compatible part that simply codes for two stop codons. This part does not have any degradation tag.
false
true
_41_
0
6384
41
Not in stock
false
Made with synthetic oligos:
5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3'
5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3'
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
false
Joseph Lynch
annotation2071257
1
stop
range2071257
1
1
5
BBa_K1362441
1
Xyla
Xylanase (cds only)
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
???
???
false
false
_1738_
0
22920
9
Not in stock
false
???
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2407315
1
Xylanase
range2407315
1
1
555
BBa_G0000
1
scar
SpeI/XbaI scar for RBS-CDS junctions
2007-07-22T11:00:00Z
2015-08-31T04:07:27Z
SpeI/XbaI scar
This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
false
true
_41_
0
126
162
Not in stock
false
This is a shorter scar to ensure proper spacing between the RBS and CDS.
false
Reshma Shetty
BBa_K1362023_sequence
1
aaagaggagaaatactagatggctagcacagactactggcaaaattggactgatgggggcggtatagtaaacgctgtcaatgggtctggcgggaattacagtgttaattggtctaataccggaaattttgttgttggtaaaggttggactacaggttcgccatttaggacgataaactataatgccggagtttgggcgccgaatggcaatggatatttaactttatatggttggacgagatcacctctcatagaatattatgtagtggattcatggggtacttatagacctactggaacgtataaaggtactgtaaaaagtgatgggggtacatatgacatatatacaactacacgttataacgcaccttccattgatggcgatcgcactacttttacgcagtactggagtgttcgccagtcgaagagaccaaccggaagcaacgctacaatcactttcagcaatcatgtgaacgcatggaagagccatggaatgaatctgggcagtaattgggcttaccaagtcatggcgacagaaggatatcaaagtagtggaagttctaacgtaacagtgtggggcagccatcaccatcaccatcatagtggttaataa
BBa_J70594_sequence
1
taataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1362442_sequence
1
ggcagccatcaccatcaccatcatagtggt
BBa_G0000_sequence
1
tactag
BBa_K1362414_sequence
1
atg
BBa_K1362441_sequence
1
gctagcacagactactggcaaaattggactgatgggggcggtatagtaaacgctgtcaatgggtctggcgggaattacagtgttaattggtctaataccggaaattttgttgttggtaaaggttggactacaggttcgccatttaggacgataaactataatgccggagtttgggcgccgaatggcaatggatatttaactttatatggttggacgagatcacctctcatagaatattatgtagtggattcatggggtacttatagacctactggaacgtataaaggtactgtaaaaagtgatgggggtacatatgacatatatacaactacacgttataacgcaccttccattgatggcgatcgcactacttttacgcagtactggagtgttcgccagtcgaagagaccaaccggaagcaacgctacaatcactttcagcaatcatgtgaacgcatggaagagccatggaatgaatctgggcagtaattgggcttaccaagtcatggcgacagaaggatatcaaagtagtggaagttctaacgtaacagtgtgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z