BBa_J32015
1
PelB
PelB leader sequence; directs protein to E. coli periplasmic membrane
2006-08-24T11:00:00Z
2015-08-31T04:08:46Z
Novagen
Released HQ 2013
The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
false
true
_50_
0
495
50
In stock
true
.
true
Austen Heinz
annotation1898182
1
PelB
range1898182
1
1
66
BBa_K1362425
1
BsaI ->
BsaI restriction site for RFC[???] cloning (in part in prefix)
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
This Sequence starts with a part of the BsaI recognition site. The missing G of the recognition site can be found in the upstream BB prefix. It contains a spacer nucleotide so that BsaI will cut the top strand directly downstream and the bottom strand 4 nucleotides downstream.
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2402282
1
RFC[???] standard overhang A
range2402282
1
5
5
annotation2400588
1
BsaI site
range2400588
1
1
5
BBa_K1362058
1
BBa_K1362058
PelB leading sequence as RFC[105] N-terminal insert
2014-10-07T11:00:00Z
2015-05-08T01:10:04Z
false
false
_1738_
0
22830
9
In stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
component2409735
1
BBa_J32015
component2409733
1
BBa_K1362425
component2409738
1
BBa_K1362423
component2409737
1
BBa_K1362415
component2409736
1
BBa_K1362444
annotation2409735
1
BBa_J32015
range2409735
1
8
73
annotation2409736
1
BBa_K1362444
range2409736
1
74
91
annotation2409738
1
BBa_K1362423
range2409738
1
96
102
annotation2409737
1
BBa_K1362415
range2409737
1
92
95
annotation2409733
1
BBa_K1362425
range2409733
1
1
6
BBa_K1362415
1
RFC105 NN
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[http://2014.igem.org/Team:Heidelberg|wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362423
1
<- BsaI
BsaI reverse restriction site for RFC[105] cloning
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362444
1
BBa_K1362444
GGG linker with RGK (extein)
2014-10-07T11:00:00Z
2015-05-08T01:10:06Z
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362425_sequence
1
gtctcc
BBa_K1362058_sequence
1
gtctccgatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccggcggaggcaggggcaagtgctagagacc
BBa_K1362423_sequence
1
agagacc
BBa_K1362415_sequence
1
tgct
BBa_K1362444_sequence
1
ggcggaggcaggggcaag
BBa_J32015_sequence
1
atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z