BBa_K1362416
1
RFC105 NN*
N-terminal non-splicing overhang GGCT=Gly+1/3Leu|Ile RFC[105] overhang NN*
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang B* used to insert a protein in front of a non-splicing N-Intein, which could often serve as control for protein splicing experiments. It lies within the four bases formed by the glycine and the first base of the subsequent Leucine/Isoleucine or similar of the mutated N-terminal splicing site.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362440
1
RFC105 A
N-terminal start overhang (T)(A)-GATG=RBS+Start RFC[105] A
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362425
1
BsaI ->
BsaI restriction site for RFC[???] cloning (in part in prefix)
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
This Sequence starts with a part of the BsaI recognition site. The missing G of the recognition site can be found in the upstream BB prefix. It contains a spacer nucleotide so that BsaI will cut the top strand directly downstream and the bottom strand 4 nucleotides downstream.
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2400588
1
BsaI site
range2400588
1
1
5
annotation2402282
1
RFC[???] standard overhang A
range2402282
1
5
5
BBa_K1362061
1
BBa_K1362061
N-terminal chitin binding domain with triglycine linker and His-tag as RFC[105] insert
2014-10-08T11:00:00Z
2015-05-08T01:10:04Z
false
false
_1738_
0
22830
9
Not in stock
false
false
Jan Gleixner
component2430379
1
BBa_K1362260
component2430382
1
BBa_K1362423
component2430374
1
BBa_K1362425
component2430380
1
BBa_K1362466
component2430381
1
BBa_K1362416
component2430375
1
BBa_K1362440
annotation2430380
1
BBa_K1362466
range2430380
1
194
208
annotation2430381
1
BBa_K1362416
range2430381
1
209
212
annotation2430382
1
BBa_K1362423
range2430382
1
213
219
annotation2430374
1
BBa_K1362425
range2430374
1
1
6
annotation2430375
1
BBa_K1362440
range2430375
1
7
10
annotation2430379
1
BBa_K1362260
range2430379
1
11
193
BBa_K1362260
1
BBa_K1362260
Chitin binding domain with triglycine linker and His-tag
2014-10-08T11:00:00Z
2015-05-08T01:10:05Z
false
false
_1738_
0
22911
9
Not in stock
false
false
Elisabeth Sch??fer
annotation2424696
1
glycine linker
range2424696
1
19
27
annotation2424697
1
chitin binding domain
range2424697
1
28
183
annotation2424694
1
His-tag
range2424694
1
1
18
BBa_K1362423
1
<- BsaI
BsaI reverse restriction site for RFC[105] cloning
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362466
1
BBa_K1362466
Intein protease extein
2014-10-08T11:00:00Z
2015-05-08T01:10:06Z
false
false
_1738_
0
22830
9
Not in stock
false
false
Jan Gleixner
BBa_K1362425_sequence
1
gtctcc
BBa_K1362466_sequence
1
ctgcgtgaatctggt
BBa_K1362423_sequence
1
agagacc
BBa_K1362440_sequence
1
gatg
BBa_K1362061_sequence
1
gtctccgatgcatcatcaccatcaccacggtggaggtaccacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaactgcgtgaatctggtggctagagacc
BBa_K1362260_sequence
1
catcatcaccatcaccacggtggaggtaccacaaatcctggtgtatccgcttggcaggtcaacacagcttatactgcgggacaattggtcacatataacggcaagacgtataaatgtttgcagccccacacctccttggcaggatgggaaccatccaacgttcctgccttgtggcagcttcaa
BBa_K1362416_sequence
1
ggct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z