LOCUS       BBa_K1362103            1259 bp    DNA     linear       29-Apr-2024
DEFINITION  RBS + NpuDnaE C-intein RFC[105] non-slicing control construct 
            (with His6)
ACCESSION   BBa_K1362103
VERSION     BBa_K1362103.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..56
                     /label=His6
     misc_feature    57..59
                     /label=Gly
     misc_feature    39..59
                     /label=BBa_K1362431
     misc_feature    60..63
                     /label=BBa_K1362418
     misc_feature    64..65
                     /label=2/3Gly
     misc_feature    64..65
                     /label=BBa_K1362464
     CDS             66..166
                     /label=NpuDnaE C-Intein
     misc_feature    164..164
                     /label=to prevent formation of SpeI site
     misc_feature    66..166
                     /label=BBa_K1362399
     misc_feature    167..170
                     /label=BBa_K1362420
     misc_feature    171..177
                     /label=BBa_K1362423
     CDS             178..265
                     /label=end of LacI coding region (inactive)
     misc_feature    178..377
                     /label=BBa_R0010
     misc_feature    266..303
                     /label=CAP binding site
     promoter        314..319
                     /label=-35
     promoter        338..343
                     /label=-10
     misc_feature    343..377
                     /label=LacI binding site
     misc_feature    350..350
                     /label=start
     promoter        178..377
                     /label=BBa_R0010
     misc_feature    390..393
                     /label=conserved
     RBS             386..397
                     /label=BBa_B0034
     CDS             404..1078
                     /label=mrfp1
     misc_feature    1085..1109
                     /label=Help:Barcodes
     CDS             404..1084
                     /label=BBa_E1010
     stem_loop       1129..1172
                     /label=stem_loop
     misc_feature    1118..1197
                     /label=BBa_B0010
     terminator      1118..1197
                     /label=BBa_B0010
     stem_loop       1213..1232
                     /label=T7 TE
     misc_feature    1239..1239
                     /label=stop
     polyA_site      1233..1246
                     /label=polya
     misc_feature    1206..1246
                     /label=BBa_B0012
     terminator      1206..1246
                     /label=BBa_B0012
     misc_feature    178..1246
                     /label=BBa_J04450
     misc_feature    1247..1253
                     /label=BBa_K1362424
     misc_feature    171..1253
                     /label=BBa_K1362422
     misc_feature    1254..1257
                     /label=stop
     misc_feature    1254..1257
                     /label=BBa_K1362421
     misc_feature    1258..1259
                     /label=BBa_K1362465
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgca tcatcaccat caccacggtg
       61 gtggaatcaa aatagccaca cgtaaatatt taggcaaaca aaatgtctat gacattggag
      121 ttgagcgcga ccataatttt gcactcaaaa atggcttcat agcagcaggc agagacccaa
      181 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      241 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      301 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      361 gataacaatt tcacacatac tagagaaaga ggagaaatac tagatggctt cctccgaaga
      421 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
      481 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
      541 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
      601 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
      661 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
      721 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
      781 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
      841 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
      901 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
      961 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
     1021 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
     1081 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
     1141 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1201 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggtc tcataataa
//