LOCUS       BBa_K1362105            1256 bp    DNA     linear       30-Apr-2024
DEFINITION  RBS + NpuDnaE C-intein RFC[105] assembly construct
ACCESSION   BBa_K1362105
VERSION     BBa_K1362105.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..139
                     /label=BBa_K1362401
     misc_feature    140..143
                     /label=BBa_K1362419
     misc_feature    144..150
                     /label=BBa_K1362423
     CDS             151..238
                     /label=end of LacI coding region (inactive)
     misc_feature    151..350
                     /label=BBa_R0010
     misc_feature    239..276
                     /label=CAP binding site
     promoter        287..292
                     /label=-35
     promoter        311..316
                     /label=-10
     misc_feature    316..350
                     /label=LacI binding site
     misc_feature    323..323
                     /label=start
     promoter        151..350
                     /label=BBa_R0010
     misc_feature    363..366
                     /label=conserved
     RBS             359..370
                     /label=BBa_B0034
     CDS             377..1051
                     /label=mrfp1
     misc_feature    1058..1082
                     /label=Help:Barcodes
     CDS             377..1057
                     /label=BBa_E1010
     stem_loop       1102..1145
                     /label=stem_loop
     misc_feature    1091..1170
                     /label=BBa_B0010
     terminator      1091..1170
                     /label=BBa_B0010
     stem_loop       1186..1205
                     /label=T7 TE
     misc_feature    1212..1212
                     /label=stop
     polyA_site      1206..1219
                     /label=polya
     misc_feature    1179..1219
                     /label=BBa_B0012
     terminator      1179..1219
                     /label=BBa_B0012
     misc_feature    151..1219
                     /label=BBa_J04450
     misc_feature    1220..1226
                     /label=BBa_K1362424
     misc_feature    144..1226
                     /label=BBa_K1362422
     misc_feature    1227..1230
                     /label=stop
     misc_feature    1227..1230
                     /label=BBa_K1362421
     misc_feature    1254..1256
                     /label=stop
     misc_feature    1251..1253
                     /label=stop
     misc_feature    1233..1250
                     /label=iGEMHD
     misc_feature    1231..1256
                     /label=BBa_K1362999
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgat caaaatagcc acacgtaaat
       61 atttaggcaa acaaaatgtc tatgacattg gagttgagcg cgaccataat tttgcactca
      121 aaaatggctt catagcttcc aacagagacc caatacgcaa accgcctctc cccgcgcgtt
      181 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      241 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      301 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa
      361 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
      421 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
      481 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
      541 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
      601 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
      661 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
      721 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
      781 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
      841 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
      901 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
      961 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
     1021 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc
     1081 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1141 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1201 gggcctttct gcgtttatag gtctcataat aaatcggtga aatgcacgac tgatag
//