LOCUS BBa_K1362107 2322 bp DNA linear 06-May-2024 DEFINITION RBS + NpuDnaE C-intein RFC[105] assembly construct with additional N-teminal insertion site ACCESSION BBa_K1362107 VERSION BBa_K1362107.1 FEATURES Location/Qualifiers RBS 11..28 /label=t7 RBS misc_feature 21..28 /label=Shine-Dalgarno RBS 1..29 /label=BBa_K1362090 misc_feature 30..35 /label=BBa_G0000 misc_feature 36..38 /label=BBa_K1362414 misc_feature 39..45 /label=BBa_K1362423 CDS 46..133 /label=end of LacI coding region (inactive) misc_feature 46..245 /label=BBa_R0010 misc_feature 134..171 /label=CAP binding site promoter 182..187 /label=-35 promoter 206..211 /label=-10 misc_feature 211..245 /label=LacI binding site misc_feature 218..218 /label=start promoter 46..245 /label=BBa_R0010 misc_feature 258..261 /label=conserved RBS 254..265 /label=BBa_B0034 CDS 272..946 /label=mrfp1 misc_feature 953..977 /label=Help:Barcodes CDS 272..952 /label=BBa_E1010 stem_loop 997..1040 /label=stem_loop misc_feature 986..1065 /label=BBa_B0010 terminator 986..1065 /label=BBa_B0010 stem_loop 1081..1100 /label=T7 TE misc_feature 1107..1107 /label=stop polyA_site 1101..1114 /label=polya misc_feature 1074..1114 /label=BBa_B0012 terminator 1074..1114 /label=BBa_B0012 misc_feature 46..1114 /label=BBa_J04450 misc_feature 1115..1121 /label=BBa_K1362424 misc_feature 39..1121 /label=BBa_K1362422 misc_feature 1122..1125 /label=BBa_K1362418 misc_feature 1126..1127 /label=2/3Gly misc_feature 1126..1127 /label=BBa_K1362464 misc_feature 1128..1228 /label=BBa_K1362401 misc_feature 1229..1232 /label=BBa_K1362419 misc_feature 1233..1239 /label=BBa_K1362423 CDS 1240..1327 /label=end of LacI coding region (inactive) misc_feature 1240..1439 /label=BBa_R0010 misc_feature 1328..1365 /label=CAP binding site promoter 1376..1381 /label=-35 promoter 1400..1405 /label=-10 misc_feature 1405..1439 /label=LacI binding site misc_feature 1412..1412 /label=start promoter 1240..1439 /label=BBa_R0010 misc_feature 1452..1455 /label=conserved RBS 1448..1459 /label=BBa_B0034 CDS 1466..2140 /label=mrfp1 misc_feature 2147..2171 /label=Help:Barcodes CDS 1466..2146 /label=BBa_E1010 stem_loop 2191..2234 /label=stem_loop misc_feature 2180..2259 /label=BBa_B0010 terminator 2180..2259 /label=BBa_B0010 stem_loop 2275..2294 /label=T7 TE misc_feature 2301..2301 /label=stop polyA_site 2295..2308 /label=polya misc_feature 2268..2308 /label=BBa_B0012 terminator 2268..2308 /label=BBa_B0012 misc_feature 1240..2308 /label=BBa_J04450 misc_feature 2309..2315 /label=BBa_K1362424 misc_feature 1233..2315 /label=BBa_K1362422 misc_feature 2316..2319 /label=stop misc_feature 2316..2319 /label=BBa_K1362421 misc_feature 2320..2321 /label=stop misc_feature 2320..2322 /label=BBa_K1362437 ORIGIN 1 aataattttg tttaacttta agaaggagat actagatgag agacccaata cgcaaaccgc 61 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 241 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 301 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 361 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 421 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 481 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 541 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 601 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 661 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 721 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 781 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 841 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 901 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 961 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1081 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc aggtggaatc aaaatagcca 1141 cacgtaaata tttaggcaaa caaaatgtct atgacattgg agttgagcgc gaccataatt 1201 ttgcactcaa aaatggcttc atagcttcca acagagaccc aatacgcaaa ccgcctctcc 1261 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 1321 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 1381 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat 1441 actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat 1501 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga 1561 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg 1621 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta 1681 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa 1741 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc 1801 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga 1861 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc 1921 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca 1981 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc 2041 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga 2101 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta 2161 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2221 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2281 ccttcgggtg ggcctttctg cgtttatagg tctcataata aa //