LOCUS       BBa_K1362150            1533 bp    DNA     linear       04-May-2024
DEFINITION  RBS + SspDnaE N-intein RFC[105] assembly construct (with His6)
ACCESSION   BBa_K1362150
VERSION     BBa_K1362150.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..45
                     /label=BBa_K1362423
     CDS             46..133
                     /label=end of LacI coding region (inactive)
     misc_feature    46..245
                     /label=BBa_R0010
     misc_feature    134..171
                     /label=CAP binding site
     promoter        182..187
                     /label=-35
     promoter        206..211
                     /label=-10
     misc_feature    211..245
                     /label=LacI binding site
     misc_feature    218..218
                     /label=start
     promoter        46..245
                     /label=BBa_R0010
     misc_feature    258..261
                     /label=conserved
     RBS             254..265
                     /label=BBa_B0034
     CDS             272..946
                     /label=mrfp1
     misc_feature    953..977
                     /label=Help:Barcodes
     CDS             272..952
                     /label=BBa_E1010
     stem_loop       997..1040
                     /label=stem_loop
     misc_feature    986..1065
                     /label=BBa_B0010
     terminator      986..1065
                     /label=BBa_B0010
     stem_loop       1081..1100
                     /label=T7 TE
     misc_feature    1107..1107
                     /label=stop
     polyA_site      1101..1114
                     /label=polya
     misc_feature    1074..1114
                     /label=BBa_B0012
     terminator      1074..1114
                     /label=BBa_B0012
     misc_feature    46..1114
                     /label=BBa_J04450
     misc_feature    1115..1121
                     /label=BBa_K1362424
     misc_feature    39..1121
                     /label=BBa_K1362422
     misc_feature    1122..1125
                     /label=BBa_K1362415
     misc_feature    1126..1496
                     /label=BBa_K1362410
     misc_feature    1499..1502
                     /label=RFC[???] C
     misc_feature    1497..1502
                     /label=GlyGly
     misc_feature    1497..1502
                     /label=BBa_K1362417
     misc_feature    1506..1523
                     /label=His6
     misc_feature    1524..1526
                     /label=Stop
     misc_feature    1503..1526
                     /label=BBa_K1362430
     misc_feature    1527..1530
                     /label=stop
     misc_feature    1527..1530
                     /label=BBa_K1362421
     misc_feature    1531..1532
                     /label=stop
     misc_feature    1531..1533
                     /label=BBa_K1362437
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgag agacccaata cgcaaaccgc
       61 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      241 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga
      301 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
      361 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
      421 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
      481 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
      541 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga
      601 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
      661 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
      721 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
      781 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
      841 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
      901 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata
      961 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1081 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc atgcttaagt tttggtaccg
     1141 aaattttaac cgttgagtac ggcccattgc ccattggcaa aattgtgagt gaagaaatta
     1201 attgttctgt gtacagtgtt gatccagaag ggagagttta cacccaggcg atcgcccaat
     1261 ggcatgaccg gggagagcag gaagtattgg aatatgaatt ggaagatggt tcagtaatcc
     1321 gagctacctc tgaccaccgc tttttaacca ccgattatca actgttggcg atcgaagaaa
     1381 tttttgctag gcaactggac ttgttgactt tagaaaatat taagcaaact gaagaagctc
     1441 ttgacaacca tcgtcttccc tttccattac ttgacgcagg tacaattaaa tcccgtggtg
     1501 gtggtcatca tcaccatcac cattagtaat aaa
//