LOCUS       BBa_K1362161            1265 bp    DNA     linear       30-Apr-2024
DEFINITION  RBS + Gp41-1 C-intein RFC[105] assembly construct (with His6)
ACCESSION   BBa_K1362161
VERSION     BBa_K1362161.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..56
                     /label=His6
     misc_feature    57..59
                     /label=Gly
     misc_feature    39..59
                     /label=BBa_K1362431
     misc_feature    60..63
                     /label=BBa_K1362418
     misc_feature    64..65
                     /label=BBa_K1362467
     misc_feature    66..172
                     /label=BBa_K1362413
     misc_feature    173..176
                     /label=BBa_K1362419
     misc_feature    177..183
                     /label=BBa_K1362423
     CDS             184..271
                     /label=end of LacI coding region (inactive)
     misc_feature    184..383
                     /label=BBa_R0010
     misc_feature    272..309
                     /label=CAP binding site
     promoter        320..325
                     /label=-35
     promoter        344..349
                     /label=-10
     misc_feature    349..383
                     /label=LacI binding site
     misc_feature    356..356
                     /label=start
     promoter        184..383
                     /label=BBa_R0010
     misc_feature    396..399
                     /label=conserved
     RBS             392..403
                     /label=BBa_B0034
     CDS             410..1084
                     /label=mrfp1
     misc_feature    1091..1115
                     /label=Help:Barcodes
     CDS             410..1090
                     /label=BBa_E1010
     stem_loop       1135..1178
                     /label=stem_loop
     misc_feature    1124..1203
                     /label=BBa_B0010
     terminator      1124..1203
                     /label=BBa_B0010
     stem_loop       1219..1238
                     /label=T7 TE
     misc_feature    1245..1245
                     /label=stop
     polyA_site      1239..1252
                     /label=polya
     misc_feature    1212..1252
                     /label=BBa_B0012
     terminator      1212..1252
                     /label=BBa_B0012
     misc_feature    184..1252
                     /label=BBa_J04450
     misc_feature    1253..1259
                     /label=BBa_K1362424
     misc_feature    177..1259
                     /label=BBa_K1362422
     misc_feature    1260..1263
                     /label=stop
     misc_feature    1260..1263
                     /label=BBa_K1362421
     misc_feature    1264..1265
                     /label=BBa_K1362465
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgca tcatcaccat caccacggtg
       61 gtggtatgat gctgaaaaaa atcctgaaaa tcgaagagct ggatgaacgt gaactgatcg
      121 atattgaggt gtccggtaac cacctgtttt acgctaacga tattctgacc cacaacagag
      181 acccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      241 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      301 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      361 tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tggcttcctc
      421 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      481 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      541 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      601 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      661 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      721 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      781 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      841 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      901 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      961 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     1021 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     1081 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
     1141 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1201 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggtctcat
     1261 aataa
//