LOCUS       BBa_K1362171             707 bp    DNA     linear       07-May-2024
DEFINITION  Split sfGFP reconstitution C-terminal splicing half
ACCESSION   BBa_K1362171
VERSION     BBa_K1362171.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=start codon
     misc_feature    36..38
                     /label=BBa_K371056
     misc_feature    39..56
                     /label=PolyHis
     misc_feature    39..56
                     /label=BBa_J18909
     misc_feature    57..157
                     /label=BBa_K1362401
     CDS             158..161
                     /label=NpuDnaE(C)
     misc_feature    158..161
                     /label=BBa_K1362477
     misc_feature    702..707
                     /label=stop
     misc_feature    684..701
                     /label=His6
     misc_feature    162..683
                     /label=sfGFP(65-238)
     misc_feature    162..707
                     /label=BBa_K1362472
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgca tcatcatcat catcatatca
       61 aaatagccac acgtaaatat ttaggcaaac aaaatgtcta tgacattgga gttgagcgcg
      121 accataattt tgcactcaaa aatggcttca tagcttctaa ttgctatggt gttcagtgct
      181 ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag
      241 gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg
      301 aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta
      361 aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgttt
      421 acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg
      481 tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg
      541 gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg tctaaagatc
      601 cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc
      661 atggtatgga tgaactgtac aaacaccacc accaccacca ctaataa
//