LOCUS       BBa_K1362202            1157 bp    DNA     linear       19-May-2024
DEFINITION  Sortase A circularization construct (with His6; without RBS)
ACCESSION   BBa_K1362202
VERSION     BBa_K1362202.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=BBa_K1362445
     misc_feature    13..33
                     /label=BBa_K1362452
     misc_feature    34..39
                     /label=BBa_K1362427
     CDS             40..127
                     /label=end of LacI coding region (inactive)
     misc_feature    40..239
                     /label=BBa_R0010
     misc_feature    128..165
                     /label=CAP binding site
     promoter        176..181
                     /label=-35
     promoter        200..205
                     /label=-10
     misc_feature    205..239
                     /label=LacI binding site
     misc_feature    212..212
                     /label=start
     promoter        40..239
                     /label=BBa_R0010
     misc_feature    252..255
                     /label=conserved
     RBS             248..259
                     /label=BBa_B0034
     CDS             266..940
                     /label=mrfp1
     misc_feature    947..971
                     /label=Help:Barcodes
     CDS             266..946
                     /label=BBa_E1010
     stem_loop       991..1034
                     /label=stem_loop
     misc_feature    980..1059
                     /label=BBa_B0010
     terminator      980..1059
                     /label=BBa_B0010
     stem_loop       1075..1094
                     /label=T7 TE
     misc_feature    1101..1101
                     /label=stop
     polyA_site      1095..1108
                     /label=polya
     misc_feature    1068..1108
                     /label=BBa_B0012
     terminator      1068..1108
                     /label=BBa_B0012
     misc_feature    40..1108
                     /label=BBa_J04450
     misc_feature    1109..1115
                     /label=BBa_K1362424
     misc_feature    1116..1133
                     /label=BBa_K1362454
     misc_feature    1134..1151
                     /label=BBa_K1362457
     misc_feature    1152..1156
                     /label=stop
     misc_feature    1152..1157
                     /label=BBa_J70594
ORIGIN
        1 atgggcagta gtgagaatct ttacttccag ggttgagacc aatacgcaaa ccgcctctcc
       61 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      121 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      181 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat
      241 actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
      301 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
      361 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
      421 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
      481 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
      541 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
      601 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
      661 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
      721 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
      781 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
      841 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
      901 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
      961 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1021 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1081 ccttcgggtg ggcctttctg cgtttatagg tctcacttcc tgaaactggt ggtcatcacc
     1141 atcaccatca ctaataa
//