LOCUS       BBa_K1362203            1442 bp    DNA     linear       19-May-2024
DEFINITION  Sortase A circularization construct (with Smt3 and His6; without 
            RBS)
ACCESSION   BBa_K1362203
VERSION     BBa_K1362203.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start codon
     misc_feature    1..3
                     /label=BBa_K371056
     misc_feature    4..297
                     /label=Smt3
     misc_feature    4..297
                     /label=BBa_K1362429
     misc_feature    298..318
                     /label=BBa_K1362453
     misc_feature    319..324
                     /label=BBa_K1362427
     CDS             325..412
                     /label=end of LacI coding region (inactive)
     misc_feature    325..524
                     /label=BBa_R0010
     misc_feature    413..450
                     /label=CAP binding site
     promoter        461..466
                     /label=-35
     promoter        485..490
                     /label=-10
     misc_feature    490..524
                     /label=LacI binding site
     misc_feature    497..497
                     /label=start
     promoter        325..524
                     /label=BBa_R0010
     misc_feature    537..540
                     /label=conserved
     RBS             533..544
                     /label=BBa_B0034
     CDS             551..1225
                     /label=mrfp1
     misc_feature    1232..1256
                     /label=Help:Barcodes
     CDS             551..1231
                     /label=BBa_E1010
     stem_loop       1276..1319
                     /label=stem_loop
     misc_feature    1265..1344
                     /label=BBa_B0010
     terminator      1265..1344
                     /label=BBa_B0010
     stem_loop       1360..1379
                     /label=T7 TE
     misc_feature    1386..1386
                     /label=stop
     polyA_site      1380..1393
                     /label=polya
     misc_feature    1353..1393
                     /label=BBa_B0012
     terminator      1353..1393
                     /label=BBa_B0012
     misc_feature    325..1393
                     /label=BBa_J04450
     misc_feature    1394..1400
                     /label=BBa_K1362424
     misc_feature    1401..1418
                     /label=BBa_K1362455
     misc_feature    1419..1436
                     /label=BBa_K1362457
     misc_feature    1437..1441
                     /label=stop
     misc_feature    1437..1442
                     /label=BBa_J70594
ORIGIN
        1 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct
       61 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa
      121 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg
      181 gactccttaa gattcttgta cgacggtatt agaatccaag ctgatcagac ccctgaagat
      241 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggatccgaa
      301 aacctgtact tccagggttg agaccaatac gcaaaccgcc tctccccgcg cgttggccga
      361 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      421 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      481 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga
      541 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      601 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      661 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      721 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      781 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
      841 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
      901 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
      961 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1021 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1081 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1141 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1201 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact
     1261 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1321 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1381 ttctgcgttt ataggtctca cttccggaaa ccggtggtca tcaccatcac catcactaat
     1441 aa
//