LOCUS       BBa_K1362204            1160 bp    DNA     linear       29-May-2024
DEFINITION  Sortase A non-circularization construct (with His6; without RBS)
ACCESSION   BBa_K1362204
VERSION     BBa_K1362204.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=BBa_K1362445
     misc_feature    13..30
                     /label=BBa_K1362458
     misc_feature    31..39
                     /label=BBa_K1362449
     misc_feature    40..60
                     /label=BBa_K1362453
     misc_feature    61..66
                     /label=BBa_K1362447
     CDS             67..154
                     /label=end of LacI coding region (inactive)
     misc_feature    67..266
                     /label=BBa_R0010
     misc_feature    155..192
                     /label=CAP binding site
     promoter        203..208
                     /label=-35
     promoter        227..232
                     /label=-10
     misc_feature    232..266
                     /label=LacI binding site
     misc_feature    239..239
                     /label=start
     promoter        67..266
                     /label=BBa_R0010
     misc_feature    279..282
                     /label=conserved
     RBS             275..286
                     /label=BBa_B0034
     CDS             293..967
                     /label=mrfp1
     misc_feature    974..998
                     /label=Help:Barcodes
     CDS             293..973
                     /label=BBa_E1010
     stem_loop       1018..1061
                     /label=stem_loop
     misc_feature    1007..1086
                     /label=BBa_B0010
     terminator      1007..1086
                     /label=BBa_B0010
     stem_loop       1102..1121
                     /label=T7 TE
     misc_feature    1128..1128
                     /label=stop
     polyA_site      1122..1135
                     /label=polya
     misc_feature    1095..1135
                     /label=BBa_B0012
     terminator      1095..1135
                     /label=BBa_B0012
     misc_feature    67..1135
                     /label=BBa_J04450
     misc_feature    1136..1142
                     /label=BBa_K1362424
     misc_feature    1143..1154
                     /label=BBa_K1362459
     misc_feature    1155..1159
                     /label=stop
     misc_feature    1155..1160
                     /label=BBa_J70594
ORIGIN
        1 atgggcagta gtcatcatca tcatcatcac agcagcggcg aaaacctgta cttccagggt
       61 agagaccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      121 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      181 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      241 ttgtgagcgg ataacaattt cacacatact agagaaagag gagaaatact agatggcttc
      301 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
      361 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
      421 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
      481 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
      541 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
      601 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
      661 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
      721 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
      781 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
      841 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
      901 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
      961 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
     1021 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1081 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataggtct
     1141 cacttccgga aacctaataa
//