LOCUS       BBa_K1362205            1487 bp    DNA     linear       29-May-2024
DEFINITION  Sortase A non-circularization construct (with Smt3 and His6; 
            without RBS)
ACCESSION   BBa_K1362205
VERSION     BBa_K1362205.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=BBa_K1362446
     misc_feature    13..30
                     /label=BBa_K1362458
     misc_feature    31..39
                     /label=BBa_K1362449
     misc_feature    40..57
                     /label=BBa_K1362456
     misc_feature    58..72
                     /label=BBa_K1362448
     misc_feature    73..366
                     /label=Smt3
     misc_feature    73..366
                     /label=BBa_K1362429
     misc_feature    367..387
                     /label=BBa_K1362453
     misc_feature    388..393
                     /label=BBa_K1362447
     CDS             394..481
                     /label=end of LacI coding region (inactive)
     misc_feature    394..593
                     /label=BBa_R0010
     misc_feature    482..519
                     /label=CAP binding site
     promoter        530..535
                     /label=-35
     promoter        554..559
                     /label=-10
     misc_feature    559..593
                     /label=LacI binding site
     misc_feature    566..566
                     /label=start
     promoter        394..593
                     /label=BBa_R0010
     misc_feature    606..609
                     /label=conserved
     RBS             602..613
                     /label=BBa_B0034
     CDS             620..1294
                     /label=mrfp1
     misc_feature    1301..1325
                     /label=Help:Barcodes
     CDS             620..1300
                     /label=BBa_E1010
     stem_loop       1345..1388
                     /label=stem_loop
     misc_feature    1334..1413
                     /label=BBa_B0010
     terminator      1334..1413
                     /label=BBa_B0010
     stem_loop       1429..1448
                     /label=T7 TE
     misc_feature    1455..1455
                     /label=stop
     polyA_site      1449..1462
                     /label=polya
     misc_feature    1422..1462
                     /label=BBa_B0012
     terminator      1422..1462
                     /label=BBa_B0012
     misc_feature    394..1462
                     /label=BBa_J04450
     misc_feature    1463..1469
                     /label=BBa_K1362424
     misc_feature    1470..1481
                     /label=BBa_K1362459
     misc_feature    1482..1486
                     /label=stop
     misc_feature    1482..1487
                     /label=BBa_J70594
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggctagca tgtcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
      121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
      181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt
      241 aaggaaatgg actccttaag attcttgtac gacggtatta gaatccaagc tgatcagacc
      301 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt
      361 ggatccgaaa acctgtactt ccagggtaga gaccaatacg caaaccgcct ctccccgcgc
      421 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      481 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      541 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga
      601 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
      661 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      721 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      781 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      841 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      901 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      961 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
     1021 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
     1081 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
     1141 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
     1201 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
     1261 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
     1321 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1381 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1441 ggtgggcctt tctgcgttta taggtctcac ttccggaaac ctaataa
//