LOCUS       BBa_K1362422            1083 bp    DNA     linear       28-Apr-2024
DEFINITION  mRFP selection marker with outward BsaI restriction sites
ACCESSION   BBa_K1362422
VERSION     BBa_K1362422.1  
FEATURES             Location/Qualifiers
     misc_feature    1..7
                     /label=BBa_K1362423
     CDS             8..95
                     /label=end of LacI coding region (inactive)
     misc_feature    8..207
                     /label=BBa_R0010
     misc_feature    96..133
                     /label=CAP binding site
     promoter        144..149
                     /label=-35
     promoter        168..173
                     /label=-10
     misc_feature    173..207
                     /label=LacI binding site
     misc_feature    180..180
                     /label=start
     promoter        8..207
                     /label=BBa_R0010
     misc_feature    220..223
                     /label=conserved
     RBS             216..227
                     /label=BBa_B0034
     CDS             234..908
                     /label=mrfp1
     misc_feature    915..939
                     /label=Help:Barcodes
     CDS             234..914
                     /label=BBa_E1010
     stem_loop       959..1002
                     /label=stem_loop
     misc_feature    948..1027
                     /label=BBa_B0010
     terminator      948..1027
                     /label=BBa_B0010
     stem_loop       1043..1062
                     /label=T7 TE
     misc_feature    1069..1069
                     /label=stop
     polyA_site      1063..1076
                     /label=polya
     misc_feature    1036..1076
                     /label=BBa_B0012
     terminator      1036..1076
                     /label=BBa_B0012
     misc_feature    8..1076
                     /label=BBa_J04450
     misc_feature    1077..1083
                     /label=BBa_K1362424
ORIGIN
        1 agagacccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
       61 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      181 attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac tagatggctt
      241 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
      301 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
      361 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
      421 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
      481 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg
      541 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
      601 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt
      661 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa
      721 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
      781 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
      841 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
      901 ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat
      961 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1021 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggtc
     1081 tca
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