BBa_K1362425 1 BsaI -> BsaI restriction site for RFC[???] cloning (in part in prefix) 2014-10-07T11:00:00Z 2015-05-08T01:10:05Z This Sequence starts with a part of the BsaI recognition site. The missing G of the recognition site can be found in the upstream BB prefix. It contains a spacer nucleotide so that BsaI will cut the top strand directly downstream and the bottom strand 4 nucleotides downstream. false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2402282 1 RFC[???] standard overhang A range2402282 1 5 5 annotation2400588 1 BsaI site range2400588 1 1 5 BBa_K1362425_sequence 1 gtctcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z