SortA clv.

BBa_K1362454 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1362454

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:06

Sortase A recognition/cleavage site

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K1362454_sequence (Version 1)

Description

This part codes for the amino acid sequence LPETGG. This site is recognized by Sortase A, which will cleave the Thr-Gly peptide bond and subsequently act as a transpeptidase to attach any peptide with an N-terminal glycine [[{{PAGENAME}}:Design#References|[1]]]. The first four nucleotides of this sequence are CTTC, which will be the overhang produced wenn a forward-facing BsaI site (BBa_K1362424, BBa_K1362425) is directly following this part, as in the Sortase A circularization constructs (BBa_K1362202, BBa_K1362203, BBa_K1362204, BBa_K1362205).

Source

'Staphylococcus aureus

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1362454/1

Member of these Collections

• iGEM Parts Registry