Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sandra Mous
Date created: 2014-09-23 11:00:00
Date modified: 2015-05-08 01:10:07
sfGFP(Bs)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1365020_sequence (Version 1) |
Description
Codes for the sfGFP protein, an optimized fluorescent reporter. Was originally optimized for Bacillus subtilis, but then showed high fluorescence in Lactococcus lactis.Notes
Used primers:forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3'
to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix.
Source
PCR on plasmid MG_sfgfp(Bs), used in the study 'Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging', see references.Plasmid kindly provided by the Molecular Genetics group of the Rijksuniversiteit Groningen.