Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:07
Genetic construct for msbB gene deletion (C2)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1366102_sequence (Version 1) |
Description
This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.Notes
This is the general structure50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp
Source
The genetic design is based in the lpp deletion done by:Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356.
It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015
| Sequence Annotation | Location | Component / Role(s) |
| msbB homology arm FRT Kan promoter RBS KanR Double terminator FRT msbB homology arm MCS | 1,50 51,85 86,131 132,144 145,940 941,1070 1071,1105 1260,1310 1106,1259 | sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter feature/rbs ribosome_entry_site feature/cds CDS stop_codon feature/stop feature/dna sequence_feature feature/misc sequence_feature feature/scar |