BBa_K1366105
1
C6
Production of survivin siRNA under the hTERT promoter (C6)
2014-10-09T11:00:00Z
2015-05-08T01:10:07Z
We designed the siRNA. And the other parts come from the iGEM registry
This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome.
false
false
_1743_
0
16721
9
It's complicated
false
The design sequence is as follows: hTERT // siRNA // terminador
false
Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu
annotation2415744
1
survivin siRNA 1
range2415744
1
460
513
annotation2415745
1
hGH polyadenylation
range2415745
1
514
995
annotation2415743
1
hTERT promoter
range2415743
1
1
459
BBa_K1366105_sequence
1
gtggcccctccctcgggttaccccacagcctaggccgattcgacctctctccgctggggccctcgctggcgtccctgcaccctgggagcgcgagcggcgcgcgggcggggaagcgcggcccagacccccgggtccgcccggagcagctgcgctgtcggggccaggccgggctcccagtggattcgcgggcacagacgcccaggaccgcgctccccacgtggcggagggactggggacccgggcacccgtcctgccccttcaccttccagctccgcctcctccgcgcggaccccgccccgtcccgacccctcccgggtccccggcccagccccctccgggccctcccagcccctccccttcctttccgcggccccgccctctcctcgcggcgcgagtttcaggcagcgctgcgtcctgctgcgcacgtgggaagccctggccccggccacccccgcgattggaccaccgcatctctacaatatttcaagagaatattgtagagatgcggtggtccgacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z