BBa_K1366105 1 C6 Production of survivin siRNA under the hTERT promoter (C6) 2014-10-09T11:00:00Z 2015-05-08T01:10:07Z We designed the siRNA. And the other parts come from the iGEM registry This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. false false _1743_ 0 16721 9 It's complicated false The design sequence is as follows: hTERT // siRNA // terminador false Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu annotation2415744 1 survivin siRNA 1 range2415744 1 460 513 annotation2415745 1 hGH polyadenylation range2415745 1 514 995 annotation2415743 1 hTERT promoter range2415743 1 1 459 BBa_K1366105_sequence 1 gtggcccctccctcgggttaccccacagcctaggccgattcgacctctctccgctggggccctcgctggcgtccctgcaccctgggagcgcgagcggcgcgcgggcggggaagcgcggcccagacccccgggtccgcccggagcagctgcgctgtcggggccaggccgggctcccagtggattcgcgggcacagacgcccaggaccgcgctccccacgtggcggagggactggggacccgggcacccgtcctgccccttcaccttccagctccgcctcctccgcgcggaccccgccccgtcccgacccctcccgggtccccggcccagccccctccgggccctcccagcccctccccttcctttccgcggccccgccctctcctcgcggcgcgagtttcaggcagcgctgcgtcctgctgcgcacgtgggaagccctggccccggccacccccgcgattggaccaccgcatctctacaatatttcaagagaatattgtagagatgcggtggtccgacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z