LOCUS       BBa_K1368003            2413 bp    DNA     linear       15-May-2024
DEFINITION  ptetR+lox66+lacI+plac(R0011)+c1
ACCESSION   BBa_K1368003
VERSION     BBa_K1368003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..96
                     /label=BBa_I718016
     misc_feature    109..112
                     /label=conserved
     RBS             105..116
                     /label=BBa_B0034
     misc_feature    1212..1250
                     /label=LVA
     CDS             123..1250
                     /label=lacI-LVA
     misc_feature    123..1250
                     /label=BBa_C0012
     misc_feature    1251..1275
                     /label=Help:Barcodes
     CDS             123..1250
                     /label=BBa_C0012
     stem_loop       1295..1338
                     /label=stem_loop
     misc_feature    1284..1363
                     /label=BBa_B0010
     terminator      1284..1363
                     /label=BBa_B0010
     stem_loop       1379..1398
                     /label=T7 TE
     misc_feature    1405..1405
                     /label=stop
     polyA_site      1399..1412
                     /label=polya
     misc_feature    1372..1412
                     /label=BBa_B0012
     terminator      1372..1412
                     /label=BBa_B0012
     misc_feature    105..1412
                     /label=BBa_P0412
     misc_feature    1423..1439
                     /label=lac O1
     promoter        1440..1445
                     /label=-35
     misc_feature    1446..1462
                     /label=lac O1
     promoter        1463..1468
                     /label=-10
     misc_feature    1421..1474
                     /label=BBa_R0011
     promoter        1421..1474
                     /label=BBa_R0011
     misc_feature    1488..1491
                     /label=conserved
     RBS             1484..1495
                     /label=BBa_B0034
     CDS             1505..2212
                     /label=cI lambda
     misc_feature    2213..2245
                     /label=LVA
     misc_feature    2252..2276
                     /label=Help:Barcodes
     CDS             1502..2251
                     /label=BBa_C0051
     stem_loop       2296..2339
                     /label=stem_loop
     misc_feature    2285..2364
                     /label=BBa_B0010
     terminator      2285..2364
                     /label=BBa_B0010
     stem_loop       2380..2399
                     /label=T7 TE
     misc_feature    2406..2406
                     /label=stop
     polyA_site      2400..2413
                     /label=polya
     misc_feature    2373..2413
                     /label=BBa_B0012
     terminator      2373..2413
                     /label=BBa_B0012
     misc_feature    1484..2413
                     /label=BBa_P0451
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agataacttg gtatagcata cattatacga acggtatact agagaaagag gagaaatact
      121 agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
      181 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
      241 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
      301 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
      361 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
      421 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
      481 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
      541 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
      601 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
      661 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
      721 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
      781 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
      841 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
      901 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
      961 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     1021 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     1081 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     1141 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1201 aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     1261 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1321 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1381 ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattgtgagc ggataacaat
     1441 tgacattgtg agcggataac aagatactga gcacatacta gagaaagagg agaaatacta
     1501 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
     1561 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
     1621 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
     1681 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
     1741 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
     1801 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
     1861 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
     1921 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
     1981 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
     2041 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
     2101 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
     2161 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa
     2221 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact
     2281 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2341 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2401 ttctgcgttt ata
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