LOCUS BBa_K137019 2855 bp DNA linear 05-May-2024 DEFINITION AHL receiver + tet inverter + GFP ACCESSION BBa_K137019 VERSION BBa_K137019.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 CDS 1092..1708 /label=tetR misc_feature 1709..1742 /label=SsrA misc_feature 1749..1773 /label=Help:Barcodes CDS 1089..1748 /label=BBa_C0040 stem_loop 1793..1836 /label=stem_loop misc_feature 1782..1861 /label=BBa_B0010 terminator 1782..1861 /label=BBa_B0010 stem_loop 1877..1896 /label=T7 TE misc_feature 1903..1903 /label=stop polyA_site 1897..1910 /label=polya misc_feature 1870..1910 /label=BBa_B0012 terminator 1870..1910 /label=BBa_B0012 misc_feature 1919..1937 /label=TetR 1 misc_feature 1919..1972 /label=BBa_R0040 promoter 1938..1943 /label=-35 misc_feature 1944..1962 /label=TetR 2 promoter 1961..1966 /label=-10 promoter 1919..1972 /label=BBa_R0040 misc_feature 1071..1972 /label=BBa_Q04400 misc_feature 1985..1988 /label=conserved RBS 1981..1992 /label=BBa_B0034 CDS 1999..2718 /label=GFP protein CDS 1999..2718 /label=BBa_E0040 stem_loop 2738..2781 /label=stem_loop misc_feature 2727..2806 /label=BBa_B0010 terminator 2727..2806 /label=BBa_B0010 stem_loop 2822..2841 /label=T7 TE misc_feature 2848..2848 /label=stop polyA_site 2842..2855 /label=polya misc_feature 2815..2855 /label=BBa_B0012 terminator 2815..2855 /label=BBa_B0012 misc_feature 1981..2855 /label=BBa_I13504 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta 1141 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc 1201 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg 1261 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt 1321 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa 1381 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag 1441 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg 1501 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa 1561 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg 1621 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag 1681 aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag 1741 cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg 1801 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1861 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc 1921 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag 1981 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 2041 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 2101 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 2161 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 2221 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 2281 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 2341 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 2401 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 2461 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 2521 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 2581 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 2641 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 2701 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2761 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2821 tggctcacct tcgggtgggc ctttctgcgt ttata //