BBa_K137011 1 folKE folKE (GTP Cyclohydrolase I + pyrophosphokinase) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis genome folKE is a bifunctional gene which encodes two necessary enzymes for folate biosynthesis (2-Amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase / GTP cyclohydrolase I). false false _187_ 0 2987 9 It's complicated false We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs. false Victoria Hsiao BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K137009 1 folB folB (dihydroneopterin aldolase) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis folB is part of the L.lactis folate gene cluster. false false _187_ 0 2987 9 It's complicated false We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs. false Victoria Hsiao BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K137056 1 BBa_K137056 folB + folKE + b0015 in psb2k3 2008-07-30T11:00:00Z 2015-05-08T01:10:09Z The two genes were cloned from the genome of lactococcus lactis subspe. IL1403 This is the complete construct of two of the five folate biosynthesis genes from L.lactis. We hope to use this plasmid to test the effects of overexpression of the folate gene cluster on total folate production in E. coli. false false _187_ 0 2987 9 Not in stock false The rest of the plasmid includes the constitutive promoter j23100 and the strong RBS b0034 in the inducible copy plasmid psb2k3. false Victoria Hsiao component1969255 1 BBa_B0034 component1969259 1 BBa_K137011 component1969256 1 BBa_K137009 component1969258 1 BBa_B0034 component1969253 1 BBa_J23100 component1969260 1 BBa_B0010 component1969262 1 BBa_B0012 annotation1969262 1 BBa_B0012 range1969262 1 1591 1631 annotation1969256 1 BBa_K137009 range1969256 1 62 415 annotation1969255 1 BBa_B0034 range1969255 1 44 55 annotation1969253 1 BBa_J23100 range1969253 1 1 35 annotation1969260 1 BBa_B0010 range1969260 1 1503 1582 annotation1969258 1 BBa_B0034 range1969258 1 424 435 annotation1969259 1 BBa_K137011 range1969259 1 442 1494 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K137056_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtacaaaataaaacttaataatatgaaatttagagcacatattggtgttctgccagaagaaaaagttctcggacaaaatctcgaaattgatttaatcgtggaaacaaattttgatttttcaggaaaagacgaattagatgaaactttgtcttatgttgatttctatgaggcaacaaaagcagttgtagaatcttcaaaagctgatttaattgaacatgttgcctttgaaattattcaagcagtaaaggctacttcagagcgtatatcaacggttgaagtccatcttagaaaattagccgtaccgattgaaggaatttttgattcagctgaaatcgagatgagagggtaataatactagagaaagaggagaaatactagatgcaaacaacttatttaagcatgggaagtaatattggtgaccgtcagtattatttacatgaagccattcgtttattgggaaaacaccctaaaattatgattgaaaaagtatcaaatttttatgaaagtactccagtcggcggcgtcaaacaagatgattttactaatttggcattaaaggtggcaacgctacttgaacctttggaattattatcttttattcatgaagttgagctatctttgaaccgtgagcgaaaaattcattgggggccaagaacaattgatattgatattattttctataacgacttagaaatgcaagaagaaaacttggttattccacataaagaagcttttaatcgtctttttgtcttgaaacctatttttgaacttattgataaagactttaaatattatgcgtcaatagaaaaagcaatagccgaactttcagtaagtgaacaagagctccatgtgataaaagaagaaaaaacaccaagaaatcgtattgaagatgctgttaaagagattctctttgcagtaggtgaaaatccaaatcgagaaggattacttgaaactccagcgagagtagctaaaatgtatgaagaaattctttcgtcacaacgcttaagcaagtttaatgagtataaactttttgaaattgattcttctaaaacggattcaatcgtgttgattaaagatattcctttttattcaatgtgtgagcatcatatgttaccattttttgggaaagctcatgttgcctatattccagctgatggaaaaattattggcttgtcaaaaattccccgtttagttgattatgtttcgcgcaaactctcagttcaagaaaatatcactcatgatattggagatgttttgactgatattttgaatcctaaaggagtggcagttcttgttgaaggacgtcatatgtgcgttgaaatgcgtggagtaaaaaaagtaaattctattactaaaacttcttattttttaggtgaatttaaagaaaataatgaaaaaagaatggaatttttagaaagtcttttataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K137009_sequence 1 atgtacaaaataaaacttaataatatgaaatttagagcacatattggtgttctgccagaagaaaaagttctcggacaaaatctcgaaattgatttaatcgtggaaacaaattttgatttttcaggaaaagacgaattagatgaaactttgtcttatgttgatttctatgaggcaacaaaagcagttgtagaatcttcaaaagctgatttaattgaacatgttgcctttgaaattattcaagcagtaaaggctacttcagagcgtatatcaacggttgaagtccatcttagaaaattagccgtaccgattgaaggaatttttgattcagctgaaatcgagatgagagggtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K137011_sequence 1 atgcaaacaacttatttaagcatgggaagtaatattggtgaccgtcagtattatttacatgaagccattcgtttattgggaaaacaccctaaaattatgattgaaaaagtatcaaatttttatgaaagtactccagtcggcggcgtcaaacaagatgattttactaatttggcattaaaggtggcaacgctacttgaacctttggaattattatcttttattcatgaagttgagctatctttgaaccgtgagcgaaaaattcattgggggccaagaacaattgatattgatattattttctataacgacttagaaatgcaagaagaaaacttggttattccacataaagaagcttttaatcgtctttttgtcttgaaacctatttttgaacttattgataaagactttaaatattatgcgtcaatagaaaaagcaatagccgaactttcagtaagtgaacaagagctccatgtgataaaagaagaaaaaacaccaagaaatcgtattgaagatgctgttaaagagattctctttgcagtaggtgaaaatccaaatcgagaaggattacttgaaactccagcgagagtagctaaaatgtatgaagaaattctttcgtcacaacgcttaagcaagtttaatgagtataaactttttgaaattgattcttctaaaacggattcaatcgtgttgattaaagatattcctttttattcaatgtgtgagcatcatatgttaccattttttgggaaagctcatgttgcctatattccagctgatggaaaaattattggcttgtcaaaaattccccgtttagttgattatgtttcgcgcaaactctcagttcaagaaaatatcactcatgatattggagatgttttgactgatattttgaatcctaaaggagtggcagttcttgttgaaggacgtcatatgtgcgttgaaatgcgtggagtaaaaaaagtaaattctattactaaaacttcttattttttaggtgaatttaaagaaaataatgaaaaaagaatggaatttttagaaagtcttttataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z