LOCUS       BBa_K137059             1319 bp    DNA     linear       19-May-2024
DEFINITION  GFP fimE switch with 350 bp tetR promoter
ACCESSION   BBa_K137059
VERSION     BBa_K137059.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=IRL in
     misc_feature    19..35
                     /label=IRL out
     misc_feature    1..35
                     /label=BBa_K137010
     promoter        44..97
                     /label=tetR promoter
     promoter        44..393
                     /label=BBa_K137048
     misc_feature    402..418
                     /label=IRR out
     misc_feature    420..436
                     /label=IRR in
     misc_feature    402..436
                     /label=BBa_K137008
     misc_feature    449..452
                     /label=conserved
     RBS             445..456
                     /label=BBa_B0034
     CDS             463..1182
                     /label=GFP protein
     CDS             463..1182
                     /label=BBa_E0040
     stem_loop       1202..1245
                     /label=stem_loop
     misc_feature    1191..1270
                     /label=BBa_B0010
     terminator      1191..1270
                     /label=BBa_B0010
     stem_loop       1286..1305
                     /label=T7 TE
     misc_feature    1312..1312
                     /label=stop
     polyA_site      1306..1319
                     /label=polya
     misc_feature    1279..1319
                     /label=BBa_B0012
     terminator      1279..1319
                     /label=BBa_B0012
ORIGIN
        1 tctatgagtc aaaatggccc caattgtctt gtatttacta gaggtgctca gtatctctat
       61 cactgatagg gatgtcaatc tctatcactg atagggactc tagtatataa acgcagaaag
      121 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
      181 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
      241 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt tttcgtcgtt
      301 tgcagcggac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      361 gccgaataag aaggctggct ctgcaccttg gtgtactaga gaagatgaaa catttggggc
      421 caaactgtcc atattatact agagaaagag gagaaatact agatgcgtaa aggagaagaa
      481 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      541 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      601 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
      661 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
      721 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      781 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      841 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      901 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
      961 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     1021 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     1081 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     1141 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca
     1201 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1261 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//