LOCUS       BBa_K137101             1565 bp    DNA     linear       08-Jun-2024
DEFINITION  Strong promoter + strong RBS + tetA with (AGTC)10 repeat after 
            start codon + double terminator
ACCESSION   BBa_K137101
VERSION     BBa_K137101.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1428
                     /label=BBa_K137099
     stem_loop       1448..1491
                     /label=stem_loop
     misc_feature    1437..1516
                     /label=BBa_B0010
     terminator      1437..1516
                     /label=BBa_B0010
     stem_loop       1532..1551
                     /label=T7 TE
     misc_feature    1558..1558
                     /label=stop
     polyA_site      1552..1565
                     /label=polya
     misc_feature    1525..1565
                     /label=BBa_B0012
     terminator      1525..1565
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgagtcag tcagtcagtc agtcagtcag tcagtcagtc aaatctaaca atgcgctcat
      121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact
      181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct
      241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga
      301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc
      361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat
      421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga
      481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt
      601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga
      661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg
      721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt
      781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
      841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg
      901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc
      961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat
     1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt
     1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc
     1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg
     1261 tgcatggagc cgggccacct cgacctaata atactagagc caggcatcaa ataaaacgaa
     1321 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1381 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagccag
     1441 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1501 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1561 ttata
//