LOCUS       BBa_K137110             2699 bp    DNA     linear       01-Jun-2024
DEFINITION  pabA + pabB complete pABA construct
ACCESSION   BBa_K137110
VERSION     BBa_K137110.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..646
                     /label=BBa_K137005
     misc_feature    659..662
                     /label=conserved
     RBS             655..666
                     /label=BBa_B0034
     misc_feature    673..673
                     /label=Changed T to A 
     CDS             673..2562
                     /label=BBa_K137006
     stem_loop       2582..2625
                     /label=stem_loop
     misc_feature    2571..2650
                     /label=BBa_B0010
     terminator      2571..2650
                     /label=BBa_B0010
     stem_loop       2666..2685
                     /label=T7 TE
     misc_feature    2692..2692
                     /label=stop
     polyA_site      2686..2699
                     /label=polya
     misc_feature    2659..2699
                     /label=BBa_B0012
     terminator      2659..2699
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaattg ctattaattg ataattatga ttcttttact tatttgcttg cacagtattt
      121 tgaggaatta gattgtagtg taacagtggt taatgaccaa gataaaatga gtcagaaaat
      181 tagaatttct ccggatttta tttgtgaaaa ttatgatgcg attatcattt ctccaggtcc
      241 aaaaacacca aaagaagccg tttttagtag ggatgtagtt caactatacg ctggaaaaat
      301 tcctatgtta ggaatttgct tgggtcagca agtcattgct gaatgttttg gaggaaatgt
      361 tgttcttggt gagaggccaa tgcatggaaa aatttctgtc attcgtcata attatcaagg
      421 aatttttaaa ggacttccac aaaatttgaa agttgcacgt tatcattcgt tgattgtcga
      481 taaactacct gatgattttg aaattgatgc gcagagtgag gatggtgtta ttcaggccat
      541 tcatcagcca aaattaaaat tgtgggcttt acaatttcac ccagaaagtt tagttactga
      601 atatggtcat gaaatgttaa ataatttttt gaaagtggtg taataatact agagaaagag
      661 gagaaatact agatgaagga atttataata aaaaatacgg atatttggaa aattttccta
      721 aaatattatc ggtcagatga agaaattgtt tttttacata gtagtcaggt gaccgaaaat
      781 gagcattata gtattttagc tcataaacct tataaaaagg tttcaaaata taagggtcaa
      841 gtgtttttta atggtgaaaa aaagaagttc aattttttag atgcggtaga tttgctgaag
      901 gatgagagag ttgaacgacc taaaaattgg cctttttatc cagaattatt gggttttgta
      961 agttatgagc aagatccggc ttgttttgca gcttatgatg aagttttact ttttgaccat
     1021 agaacaaaac tgttacgtgt cgtgcaattt gaacaaactg atggccaata ttggctgact
     1081 gaatcggaag aaattgaagt ggattcagag attgagttcg atgttcagaa tggcataggg
     1141 gctgttttta ttgaccaaac gaggcaagaa tatattgctt caataaaaaa aatacaagat
     1201 tatatgaagg ctggcgatat ttatgttgcc aatttgacgc aacaatttga aatttggtcc
     1261 gatcaaaaac ctattgatgt ttttaagaaa acaaggaaac aaattcctgc tcctttctca
     1321 tcatttttac aatatcctga atggaaaatg acgcaaattt caagttctgt tgaacgcttt
     1381 gtttctattc ataatggagc attaatttca aaacccatta aaggaacaat tgccagagga
     1441 gaaaatgttg tagctgatag attacaaaag aaaatacttt ccgacagtag taaagaacgg
     1501 tctgagttac tgatggtgac ggatttattg cgtaatgata ttgcccgaat tagtcaacct
     1561 ttttctttgt ctgtccctaa atttgcagaa attgaaactt tttctcatgt tcatcaactg
     1621 gtgacttcaa taaaaagtag aattaaagaa gatttgactt tttcagaatt tatgacggct
     1681 ttatttccgg gaggttcaat cacaggtaca cctaagaagc gggcaatgga gattattaag
     1741 gaagttgaaa aacaacctag aggaatttat acagggatgc agggttggtt aagtcgagaa
     1801 atggatttgg atatgaacat cgtgattcgg acattagttc atgacggtga gcattatcag
     1861 cttggtgtag gcggtggaat tacgttcgag agcgaagcag aagcagagtt ttcagagatt
     1921 ttactaaaag caaaaccctt tttagatata ttaggcctga aagatgttcc aagtatttta
     1981 tttacaacag gccttgttaa aaatggtgaa ttgttaaatt tagagggtca tgtcaatcgc
     2041 ttgaaaaaac aataccatca tgcagatttg gaagaaaaat tgagaatatt tgcccaaaag
     2101 gttactgacg gggttttacg tatcagtact gacggagatt ctttgacccc tgggattcgt
     2161 cagttgaccc attcaaatga ggcctatcga gttaaactgt cgtcaattaa tgacaaacct
     2221 agtcttttgt ctaactttaa actttcaggc ccagattttc aaaaggtctt tcgtcaggaa
     2281 gtcttagaag cgaaaaaaga agggtttcaa gatattctat ttcatacaga tggtttgatc
     2341 agtgaattat caatcggaaa ttttgtggca aaaaagggga atcagtacga aacgccagcc
     2401 aaatatgctc taaaaggaac ttttttagat ttatttgcga aaaatcatac gctaatatat
     2461 aaagatattg cgatttctga tttgaaaact tacgatcgtt tttatatgac aaatgctgtc
     2521 agaggattag ttgaaataaa aattgatgga atttcctaat aatactagag ccaggcatca
     2581 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2641 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//